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Structure paper

TitleMycobacterium tuberculosis Phe-tRNA synthetase: structural insights into tRNA recognition and aminoacylation.
Journal, issue, pagesNucleic Acids Res., Vol. 49, Page 5351-5368, Year 2021
Publish dateSep 28, 2020 (structure data deposition date)
AuthorsMichalska, K. / Jedrzejczak, R. / Wower, J. / Chang, C. / Baragana, B. / Gilbert, I.H. / Forte, B. / Joachimiak, A.
External linksNucleic Acids Res. / PubMed:33885823
MethodsX-ray diffraction
Resolution2.19 - 2.5 Å
Structure data

PDB-7k98:
Preaminoacylation complex of M. tuberculosis PheRS with cognate precursor tRNA and 5'-O-(N-phenylalanyl)sulfamoyl-adenosine (F-AMS)
Method: X-RAY DIFFRACTION / Resolution: 2.19 Å

PDB-7k9m:
Crystal structure of the complex of M. tuberculosis PheRS with cognate precursor tRNA and 5'-O-(N-phenylalanyl)sulfamoyl-adenosine
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-7ka0:
Crystal structure of the complex of M. tuberculosis PheRS with cognate precursor tRNA and phenylalanine
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-7kab:
M. tuberculosis PheRS complex with cognate precursor tRNA and phenylalanine
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

Chemicals

ChemComp-W5Y:
5'-O-(L-phenylalanylsulfamoyl)adenosine

ChemComp-MG:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-P6G:
HEXAETHYLENE GLYCOL / precipitant*YM

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-PHE:
PHENYLALANINE

ChemComp-ACT:
ACETATE ION

ChemComp-SO4:
SULFATE ION

ChemComp-K:
Unknown entry

ChemComp-PGR:
R-1,2-PROPANEDIOL

ChemComp-PGO:
S-1,2-PROPANEDIOL

Source
  • mycobacterium tuberculosis (strain atcc 25618 / h37rv) (bacteria)
KeywordsLIGASE/RNA / aminoacylation / phenylalanyl tRNA synthetase / adenylate analog / Structural Genomics / Center for Structural Genomics of Infectious Diseases / CSGID / LIGASE-RNA complex / Ligase-tRNA complex

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