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TitleSingle particle cryo-EM of the complex between interphotoreceptor retinoid-binding protein and a monoclonal antibody.
Journal, issue, pagesFASEB J, Vol. 34, Issue 10, Page 13918-13934, Year 2020
Publish dateAug 28, 2020
AuthorsAvery E Sears / Stefan Albiez / Sahil Gulati / Benlian Wang / Philip Kiser / Lubomir Kovacik / Andreas Engel / Henning Stahlberg / Krzysztof Palczewski /
PubMed AbstractInterphotoreceptor retinoid-binding protein (IRBP) is a highly expressed protein secreted by rod and cone photoreceptors that has major roles in photoreceptor homeostasis as well as retinoid and ...Interphotoreceptor retinoid-binding protein (IRBP) is a highly expressed protein secreted by rod and cone photoreceptors that has major roles in photoreceptor homeostasis as well as retinoid and polyunsaturated fatty acid transport between the neural retina and retinal pigment epithelium. Despite two crystal structures reported on fragments of IRBP and decades of research, the overall structure of IRBP and function within the visual cycle remain unsolved. Here, we studied the structure of native bovine IRBP in complex with a monoclonal antibody (mAb5) by cryo-electron microscopy, revealing the tertiary and quaternary structure at sufficient resolution to clearly identify the complex components. Complementary mass spectrometry experiments revealed the structure and locations of N-linked carbohydrate post-translational modifications. This work provides insight into the structure of IRBP, displaying an elongated, flexible three-dimensional architecture not seen among other retinoid-binding proteins. This work is the first step in elucidation of the function of this enigmatic protein.
External linksFASEB J / PubMed:32860273 / PubMed Central
MethodsEM (single particle)
Resolution7.4 Å
Structure data

22474

7jti

EMDB-22474, PDB-7jti:
Interphotoreceptor retinoid-binding protein (IRBP) in complex with a monoclonal antibody (F3F5 mAb5)
Method: EM (single particle) / Resolution: 7.4 Å

Source
  • mus musculus (house mouse)
  • bos taurus (cattle)
  • Bovine (cattle)
KeywordsTRANSPORT PROTEIN/IMMUNE SYSTEM / IRBP / retinoid / Interphotoreceptor retinoid-binding protein / Antibody / TRANSPORT PROTEIN-IMMUNE SYSTEM complex

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