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TitleCryo-EM analysis of the HCoV-229E spike glycoprotein reveals dynamic prefusion conformational changes.
Journal, issue, pagesNat Commun, Vol. 12, Issue 1, Page 141, Year 2021
Publish dateJan 8, 2021
AuthorsXiyong Song / Yuejun Shi / Wei Ding / Tongxin Niu / Limeng Sun / Yubei Tan / Yong Chen / Jiale Shi / Qiqi Xiong / Xiaojun Huang / Shaobo Xiao / Yanping Zhu / Chongyun Cheng / Zhen F Fu / Zhi-Jie Liu / Guiqing Peng /
PubMed AbstractCoronaviruses spike (S) glycoproteins mediate viral entry into host cells by binding to host receptors. However, how the S1 subunit undergoes conformational changes for receptor recognition has not ...Coronaviruses spike (S) glycoproteins mediate viral entry into host cells by binding to host receptors. However, how the S1 subunit undergoes conformational changes for receptor recognition has not been elucidated in Alphacoronavirus. Here, we report the cryo-EM structures of the HCoV-229E S trimer in prefusion state with two conformations. The activated conformation may pose the potential exposure of the S1-RBDs by decreasing of the interaction area between the S1-RBDs and the surrounding S1-NTDs and S1-RBDs compared to the closed conformation. Furthermore, structural comparison of our structures with the previously reported HCoV-229E S structure showed that the S trimers trended to open the S2 subunit from the closed conformation to open conformation, which could promote the transition from pre- to postfusion. Our results provide insights into the mechanisms involved in S glycoprotein-mediated Alphacoronavirus entry and have implications for vaccine and therapeutic antibody design.
External linksNat Commun / PubMed:33420048 / PubMed Central
MethodsEM (single particle)
Resolution3.21 - 3.55 Å
Structure data

30496

7cyc

EMDB-30496, PDB-7cyc:
Cryo-EM structures of Alphacoronavirus spike glycoprotein
Method: EM (single particle) / Resolution: 3.21 Å

30497

7cyd

EMDB-30497, PDB-7cyd:
Cryo-EM structures of Alphacoronavirus spike glycoprotein
Method: EM (single particle) / Resolution: 3.55 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

Source
  • human coronavirus 229e
KeywordsSTRUCTURAL PROTEIN / Alphacoronavirus / spike glycoprotein

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