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TitleStructures of Class I and Class II Transcription Complexes Reveal the Molecular Basis of RamA-Dependent Transcription Activation.
Journal, issue, pagesAdv Sci (Weinh), Vol. 9, Issue 4, Page e2103669, Year 2022
Publish dateNov 10, 2021
AuthorsMin Hao / Fuzhou Ye / Milija Jovanovic / Ioly Kotta-Loizou / Qingqing Xu / Xiaohua Qin / Martin Buck / Xiaodong Zhang / Minggui Wang /
PubMed AbstractTranscription activator RamA is linked to multidrug resistance of Klebsiella pneumoniae through controlling genes that encode efflux pumps (acrA) and porin-regulating antisense RNA (micF). In ...Transcription activator RamA is linked to multidrug resistance of Klebsiella pneumoniae through controlling genes that encode efflux pumps (acrA) and porin-regulating antisense RNA (micF). In bacteria, σ , together with activators, controls the majority of genes by recruiting RNA polymerase (RNAP) to the promoter regions. RNAP and σ form a holoenzyme that recognizes -35 and -10 promoter DNA consensus sites. Many activators bind upstream from the holoenzyme and can be broadly divided into two classes. RamA acts as a class I activator on acrA and class II activator on micF, respectively. The authors present biochemical and structural data on RamA in complex with RNAP-σ at the two promoters and the data reveal the molecular basis for how RamA assembles and interacts with core RNAP and activates transcription that contributes to antibiotic resistance. Further, comparing with CAP/TAP complexes reveals common and activator-specific features in activator binding and uncovers distinct roles of the two C-terminal domains of RNAP α subunit.
External linksAdv Sci (Weinh) / PubMed:34761556 / PubMed Central
MethodsEM (single particle)
Resolution4.2 - 4.5 Å
Structure data

12156

7bef

EMDB-12156, PDB-7bef:
Structures of class II bacterial transcription complexes
Method: EM (single particle) / Resolution: 4.5 Å

12157

7beg

EMDB-12157, PDB-7beg:
Structures of class I bacterial transcription complexes
Method: EM (single particle) / Resolution: 4.2 Å

Source
  • Escherichia coli K-12 (bacteria)
  • klebsiella pneumoniae (bacteria)
  • escherichia coli (strain k12) (bacteria)
  • escherichia coli (E. coli)
KeywordsTRANSCRIPTION / antibiotic resistance / bacterial transcription activator / cryoEM

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