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TitleStructure and function of yeast Lso2 and human CCDC124 bound to hibernating ribosomes.
Journal, issue, pagesPLoS Biol, Vol. 18, Issue 7, Page e3000780, Year 2020
Publish dateJul 20, 2020
AuthorsJennifer N Wells / Robert Buschauer / Timur Mackens-Kiani / Katharina Best / Hanna Kratzat / Otto Berninghausen / Thomas Becker / Wendy Gilbert / Jingdong Cheng / Roland Beckmann /
PubMed AbstractCells adjust to nutrient deprivation by reversible translational shutdown. This is accompanied by maintaining inactive ribosomes in a hibernation state, in which they are bound by proteins with ...Cells adjust to nutrient deprivation by reversible translational shutdown. This is accompanied by maintaining inactive ribosomes in a hibernation state, in which they are bound by proteins with inhibitory and protective functions. In eukaryotes, such a function was attributed to suppressor of target of Myb protein 1 (Stm1; SERPINE1 mRNA-binding protein 1 [SERBP1] in mammals), and recently, late-annotated short open reading frame 2 (Lso2; coiled-coil domain containing short open reading frame 124 [CCDC124] in mammals) was found to be involved in translational recovery after starvation from stationary phase. Here, we present cryo-electron microscopy (cryo-EM) structures of translationally inactive yeast and human ribosomes. We found Lso2/CCDC124 accumulating on idle ribosomes in the nonrotated state, in contrast to Stm1/SERBP1-bound ribosomes, which display a rotated state. Lso2/CCDC124 bridges the decoding sites of the small with the GTPase activating center (GAC) of the large subunit. This position allows accommodation of the duplication of multilocus region 34 protein (Dom34)-dependent ribosome recycling system, which splits Lso2-containing, but not Stm1-containing, ribosomes. We propose a model in which Lso2 facilitates rapid translation reactivation by stabilizing the recycling-competent state of inactive ribosomes.
External linksPLoS Biol / PubMed:32687489 / PubMed Central
MethodsEM (single particle)
Resolution2.9 - 3.4 Å
Structure data

11096

6z6j

EMDB-11096, PDB-6z6j:
Cryo-EM structure of yeast Lso2 bound to 80S ribosomes under native condition
Method: EM (single particle) / Resolution: 3.4 Å

11097

6z6k

EMDB-11097, PDB-6z6k:
Cryo-EM structure of yeast reconstituted Lso2 bound to 80S ribosomes
Method: EM (single particle) / Resolution: 3.4 Å

11098

6z6l

EMDB-11098, PDB-6z6l:
Cryo-EM structure of human CCDC124 bound to 80S ribosomes
Method: EM (single particle) / Resolution: 3.0 Å

11099

6z6m

EMDB-11099, PDB-6z6m:
Cryo-EM structure of human 80S ribosomes bound to EBP1, eEF2 and SERBP1
Method: EM (single particle) / Resolution: 3.1 Å

11100

6z6n

EMDB-11100, PDB-6z6n:
Cryo-EM structure of human EBP1-80S ribosomes (focus on EBP1)
Method: EM (single particle) / Resolution: 2.9 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

Source
  • Saccharomyces cerevisiae ATCC 204508 / S288c (yeast)
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
  • homo sapiens (human)
  • Human (human)
KeywordsRIBOSOME / hibernation

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