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Structure paper

TitleMolecular mechanism of light-driven sodium pumping.
Journal, issue, pagesNat Commun, Vol. 11, Page 2137-2137, Year 2020
Publish dateJan 31, 2020 (structure data deposition date)
AuthorsKovalev, K. / Astashkin, R. / Gushchin, I. / Orekhov, P. / Volkov, D. / Zinovev, E. / Marin, E. / Rulev, M. / Alekseev, A. / Royant, A. ...Kovalev, K. / Astashkin, R. / Gushchin, I. / Orekhov, P. / Volkov, D. / Zinovev, E. / Marin, E. / Rulev, M. / Alekseev, A. / Royant, A. / Carpentier, P. / Vaganova, S. / Zabelskii, D. / Baeken, C. / Sergeev, I. / Balandin, T. / Bourenkov, G. / Carpena, X. / Boer, R. / Maliar, N. / Borshchevskiy, V. / Buldt, G. / Bamberg, E. / Gordeliy, V.
External linksNat Commun / PubMed:32358514
MethodsX-ray diffraction
Resolution1.8 - 2.7 Å
Structure data

PDB-6xyt:
Crystal structure of the O-state of the light-driven sodium pump KR2 in the pentameric form, pH 8.0
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-6yby:
Crystal structure of the D116N mutant of the light-driven sodium pump KR2 in the monomeric form, pH 4.6
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-6ybz:
Crystal structure of the D116N mutant of the light-driven sodium pump KR2 in the pentameric form, pH 8.0
Method: X-RAY DIFFRACTION / Resolution: 2.35 Å

PDB-6yc0:
Crystal structure of the steady-state-SMX activated state of the light-driven sodium pump KR2 in the pentameric form at room temperature, pH 8.0
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-6yc1:
Crystal structure of the H30A mutant of the light-driven sodium pump KR2 in the pentameric form, pH 8.0
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-6yc2:
Crystal structure of the light-driven sodium pump KR2 in the pentameric form at room temperature, pH 8.0
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-6yc3:
Crystal structure of the light-driven sodium pump KR2 in the pentameric form, pH 8.0
Method: X-RAY DIFFRACTION / Resolution: 2.0 Å

PDB-6yc4:
Crystal structure of the steady-state activated state of the light-driven sodium pump KR2 in the pentameric form at room temperature, pH 8.0
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

Chemicals

ChemComp-OLC:
(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate

ChemComp-LFA:
EICOSANE

ChemComp-NA:
Unknown entry

ChemComp-BOG:
octyl beta-D-glucopyranoside / detergent*YM

ChemComp-OLA:
OLEIC ACID

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-RET:
RETINAL

ChemComp-ALA:
ALANINE

Source
  • dokdonia eikasta (bacteria)
KeywordsMEMBRANE PROTEIN / rhodopsin / ion pumping / retinal / sodium pump / intermediate state

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