Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Design of multi-scale protein complexes by hierarchical building block fusion.
Journal, issue, pages	Nat Commun, Vol. 12, Issue 1, Page 2294, Year 2021
Publish date	Apr 16, 2021
Authors	Yang Hsia / Rubul Mout / William Sheffler / Natasha I Edman / Ivan Vulovic / Young-Jun Park / Rachel L Redler / Matthew J Bick / Asim K Bera / Alexis Courbet / Alex Kang / T J Brunette / Una Nattermann / Evelyn Tsai / Ayesha Saleem / Cameron M Chow / Damian Ekiert / Gira Bhabha / David Veesler / David Baker /
PubMed Abstract	A systematic and robust approach to generating complex protein nanomaterials would have broad utility. We develop a hierarchical approach to designing multi-component protein assemblies from two ...A systematic and robust approach to generating complex protein nanomaterials would have broad utility. We develop a hierarchical approach to designing multi-component protein assemblies from two classes of modular building blocks: designed helical repeat proteins (DHRs) and helical bundle oligomers (HBs). We first rigidly fuse DHRs to HBs to generate a large library of oligomeric building blocks. We then generate assemblies with cyclic, dihedral, and point group symmetries from these building blocks using architecture guided rigid helical fusion with new software named WORMS. X-ray crystallography and cryo-electron microscopy characterization show that the hierarchical design approach can accurately generate a wide range of assemblies, including a 43 nm diameter icosahedral nanocage. The computational methods and building block sets described here provide a very general route to de novo designed protein nanomaterials.
External links	Nat Commun / PubMed:33863889 / PubMed Central
Methods	EM (single particle) / X-ray diffraction
Resolution	2.4 - 19.0 Å
Structure data	22305 6xss EMDB-22305, PDB-6xss: CryoEM structure of designed helical fusion protein C4_nat_HFuse-7900 Method: EM (single particle) / Resolution: 3.7 Å EMDB-22306: CryoEM map of designed helical fusion protein C5_HFuse-3921 Method: EM (single particle) / Resolution: 8.06 Å EMDB-23168: Dihedral ring D2_Wm-01 Method: EM (single particle) / Resolution: 19.0 Å EMDB-23169: Dihedral ring D2_Wm-02 Method: EM (single particle) / Resolution: 19.0 Å EMDB-23170: Dihedral ring D2_Wm-01_trunc Method: EM (single particle) / Resolution: 19.0 Å EMDB-23171: Point Group nanocage T_Wm-1606 Method: EM (single particle) / Resolution: 19.0 Å EMDB-23172: Point Group nanocage I32_Wm-42 Method: EM (single particle) / Resolution: 9.62 Å EMDB-23173: Crown C5_Crn_HF-12_26 Method: EM (single particle) / Resolution: 6.0 Å EMDB-23174: Crown C5_Crn_HF-12_26 Method: EM (single particle) / Resolution: 6.5 Å PDB-6xh5: Hierarchical design of multi-scale protein complexes by combinatorial assembly of oligomeric helical bundle and repeat protein building blocks Method: X-RAY DIFFRACTION / Resolution: 3.32 Å PDB-6xi6: Hierarchical design of multi-scale protein complexes by combinatorial assembly of oligomeric helical bundle and repeat protein building blocks Method: X-RAY DIFFRACTION / Resolution: 2.69 Å PDB-6xns: C3_crown-05 Method: X-RAY DIFFRACTION / Resolution: 3.19 Å PDB-6xt4: C3_HD-1069 (1BH-69) - fusion protein of helical bundle and repeat protein Method: X-RAY DIFFRACTION / Resolution: 2.4 Å
Chemicals	ChemComp-HOH: WATER
Source	synthetic construct (others)
Keywords	DE NOVO PROTEIN / helical fusion / de novo design / HELICAL BUNDLE REPEAT PROTEIN / helical bundle / helical repeat