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TitlePost-Catalytic Complexes with Emtricitabine or Stavudine and HIV-1 Reverse Transcriptase Reveal New Mechanistic Insights for Nucleotide Incorporation and Drug Resistance.
Journal, issue, pagesMolecules, Vol. 25, Year 2020
Publish dateApr 27, 2020 (structure data deposition date)
AuthorsBertoletti, N. / Chan, A.H. / Schinazi, R.F. / Anderson, K.S.
External linksMolecules / PubMed:33096918
MethodsX-ray diffraction
Resolution2.53 - 2.73 Å
Structure data

PDB-6wpf:
Structure of HIV-1 Reverse Transcriptase (RT) in complex with dsDNA and d4T
Method: X-RAY DIFFRACTION / Resolution: 2.53 Å

PDB-6wph:
Structure of HIV-1 Reverse Transcriptase (RT) in complex with dsDNA and (-)-FTC
Method: X-RAY DIFFRACTION / Resolution: 2.72 Å

PDB-6wpj:
Structure of HIV-1 Reverse Transcriptase (RT) in complex with dsDNA and d4T
Method: X-RAY DIFFRACTION / Resolution: 2.73 Å

Chemicals

ChemComp-D4M:
[(5R)-5-(5-METHYL-2,4-DIOXO-3,4-DIHYDROPYRIMIDIN-1(2H)-YL)-2,5-DIHYDROFURAN-2-YL]METHYL DIHYDROGEN PHOSPHATE

ChemComp-SO4:
SULFATE ION

ChemComp-MG:
Unknown entry

ChemComp-D4T:
2',3'-DEHYDRO-2',3'-DEOXY-THYMIDINE 5'-TRIPHOSPHATE

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-43X:
[(2R,5S)-5-(4-amino-5-fluoro-2-oxopyrimidin-1(2H)-yl)-1,3-oxathiolan-2-yl]methyl dihydrogen phosphate

ChemComp-DGP:
2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE

Source
  • human immunodeficiency virus type 1 group m subtype b (isolate hxb2)
  • synthetic construct (others)
KeywordsTRANSFERASE/DNA / Reverse Transcriptase / ternary complex / d4T / chain terminator / stereochemistry / TRANSFERASE / TRANSFERASE-DNA complex / Stavudine / (-)FTC / Emtricitabine

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