EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures of human pannexin 1 reveal ion pathways and mechanism of gating.
ジャーナル・号・ページ	Nature, Vol. 584, Issue 7822, Page 646-651, Year 2020
掲載日	2020年6月3日
著者	Zheng Ruan / Ian J Orozco / Juan Du / Wei Lü /
PubMed 要旨	Pannexin 1 (PANX1) is an ATP-permeable channel with critical roles in a variety of physiological functions such as blood pressure regulation, apoptotic cell clearance and human oocyte development. ...Pannexin 1 (PANX1) is an ATP-permeable channel with critical roles in a variety of physiological functions such as blood pressure regulation, apoptotic cell clearance and human oocyte development. Here we present several structures of human PANX1 in a heptameric assembly at resolutions of up to 2.8 angström, including an apo state, a caspase-7-cleaved state and a carbenoxolone-bound state. We reveal a gating mechanism that involves two ion-conducting pathways. Under normal cellular conditions, the intracellular entry of the wide main pore is physically plugged by the C-terminal tail. Small anions are conducted through narrow tunnels in the intracellular domain. These tunnels connect to the main pore and are gated by a long linker between the N-terminal helix and the first transmembrane helix. During apoptosis, the C-terminal tail is cleaved by caspase, allowing the release of ATP through the main pore. We identified a carbenoxolone-binding site embraced by W74 in the extracellular entrance and a role for carbenoxolone as a channel blocker. We identified a gap-junction-like structure using a glycosylation-deficient mutant, N255A. Our studies provide a solid foundation for understanding the molecular mechanisms underlying the channel gating and inhibition of PANX1 and related large-pore channels.
リンク	Nature / PubMed:32494015 / PubMed Central
手法	EM (単粒子)
解像度	2.83 - 6.04 Å
構造データ	21588 6wbf EMDB-21588, PDB-6wbf: Cryo-EM structure of wild type human Pannexin 1 channel 手法: EM (単粒子) / 解像度: 2.83 Å 21589 6wbg EMDB-21589, PDB-6wbg: Cryo-EM structure of human Pannexin 1 channel with its C-terminal tail cleaved by caspase-7 手法: EM (単粒子) / 解像度: 2.97 Å 21590 6wbi EMDB-21590, PDB-6wbi: Cryo-EM structure of human Pannexin 1 channel with its C-terminal tail cleaved by caspase-7, in complex with CBX 手法: EM (単粒子) / 解像度: 4.39 Å 21591 6wbk EMDB-21591, PDB-6wbk: Cryo-EM structure of human Pannexin 1 channel with deletion of N-terminal helix and C-terminal tail 手法: EM (単粒子) / 解像度: 6.01 Å 21592 6wbl EMDB-21592, PDB-6wbl: Cryo-EM structure of human Pannexin 1 channel with deletion of N-terminal helix and C-terminal tail, in complex with CBX 手法: EM (単粒子) / 解像度: 5.13 Å 21593 6wbm EMDB-21593, PDB-6wbm: Cryo-EM structure of human Pannexin 1 channel N255A mutant 手法: EM (単粒子) / 解像度: 2.86 Å 21594 6wbn EMDB-21594, PDB-6wbn: Cryo-EM structure of human Pannexin 1 channel N255A mutant, gap junction 手法: EM (単粒子) / 解像度: 2.83 Å EMDB-21595: Cryo-EM structure of wild type human Pannexin 1 channel in the presence of EDTA 手法: EM (単粒子) / 解像度: 3.01 Å EMDB-21596: Cryo-EM structure of wild type human Pannexin 1 channel in the presence of 5 mM calcium 手法: EM (単粒子) / 解像度: 3.04 Å EMDB-21597: Cryo-EM structure of wild type human Pannexin 1 channel in the presence of 150 mM KCl 手法: EM (単粒子) / 解像度: 3.15 Å EMDB-21598: Cryo-EM structure of wild type human Pannexin 1 channel extracted using SMA 30010 手法: EM (単粒子) / 解像度: 6.04 Å
化合物	ChemComp-PTY: PHOSPHATIDYLETHANOLAMINE / リン脂質YM ChemComp-3PE: 1,2-Distearoyl-sn-glycerophosphoethanolamine / DSPE / リン脂質YM ChemComp-DGA: DIACYL GLYCEROL / 1-O,2-O-ジステアロイル-L-グリセロ-ル ChemComp-CLR: CHOLESTEROL / コレステロ-ル ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン ChemComp-CBO: CARBENOXOLONE / カルベノキソロン
由来	homo sapiens (ヒト)
キーワード	TRANSPORT PROTEIN / ion channel