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TitleA kinase bioscavenger provides antibiotic resistance by extremely tight substrate binding.
Journal, issue, pagesSci Adv, Vol. 6, Page eaaz9861-eaaz9861, Year 2020
Publish dateSep 14, 2019 (structure data deposition date)
AuthorsTerekhov, S.S. / Mokrushina, Y.A. / Nazarov, A.S. / Zlobin, A. / Zalevsky, A. / Bourenkov, G. / Golovin, A. / Belogurov Jr., A. / Osterman, I.A. / Kulikova, A.A. ...Terekhov, S.S. / Mokrushina, Y.A. / Nazarov, A.S. / Zlobin, A. / Zalevsky, A. / Bourenkov, G. / Golovin, A. / Belogurov Jr., A. / Osterman, I.A. / Kulikova, A.A. / Mitkevich, V.A. / Lou, H.J. / Turk, B.E. / Wilmanns, M. / Smirnov, I.V. / Altman, S. / Gabibov, A.G.
External linksSci Adv / PubMed:32637600
MethodsX-ray diffraction
Resolution1.35 - 2 Å
Structure data

PDB-6sui:
AMICOUMACIN KINASE AMIN
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-6sul:
Amicoumacin kinase AmiN in complex with AMP-PNP, Mg2+ and Ami
Method: X-RAY DIFFRACTION / Resolution: 1.35 Å

PDB-6sum:
Amicoumacin kinase hAmiN in complex with AMP-PNP, MG2+ and Ami
Method: X-RAY DIFFRACTION / Resolution: 1.35 Å

PDB-6sun:
Amicoumacin kinase hAmiN in complex with AMP-PNP, Ca2+ and Ami
Method: X-RAY DIFFRACTION / Resolution: 1.35 Å

PDB-6sv5:
Amicoumacin kinase AmiN in complex with ATP
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-1PE:
PENTAETHYLENE GLYCOL / precipitant*YM

ChemComp-HOH:
WATER

ChemComp-MG:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-UAM:
Amicoumacin A

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

ChemComp-ACT:
ACETATE ION

ChemComp-CA:
Unknown entry

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

Source
  • bacillus pumilus (bacteria)
  • bacillus altitudinis (bacteria)
KeywordsANTIMICROBIAL PROTEIN / KINASE / AMICOUMACIN

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