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Structure paper

TitleStructural and functional studies of SF1B Pif1 from Thermus oshimai reveal dimerization-induced helicase inhibition.
Journal, issue, pagesNucleic Acids Res., Vol. 49, Page 4129-4143, Year 2021
Publish dateJun 25, 2019 (structure data deposition date)
AuthorsDai, Y.X. / Chen, W.F. / Liu, N.N. / Teng, F.Y. / Guo, H.L. / Hou, X.M. / Dou, S.X. / Rety, S. / Xi, X.G.
External linksNucleic Acids Res. / PubMed:33784404
MethodsX-ray diffraction
Resolution1.926 - 3.787 Å
Structure data

PDB-6s3e:
Crystal structure of helicase Pif1 from Thermus oshimai in apo form
Method: X-RAY DIFFRACTION / Resolution: 3.787 Å

PDB-6s3h:
Crystal structure of helicase Pif1 from Thermus oshimai in complex with ADP-AlF4 and (dT)7ds11bp
Method: X-RAY DIFFRACTION / Resolution: 2.06 Å

PDB-6s3i:
Crystal structure of helicase Pif1 from Thermus oshimai in complex with ssDNA (dT)18 and ADP-MgF4
Method: X-RAY DIFFRACTION / Resolution: 2.455 Å

PDB-6s3m:
Crystal structure of helicase Pif1 from Thermus oshimai in complex with ssDNA (dT)18 and ADP-AlF4
Method: X-RAY DIFFRACTION / Resolution: 2.113 Å

PDB-6s3n:
Crystal structure of helicase Pif1 from Thermus oshimai in complex with ssDNA (dT)18 and ADP-VO4
Method: X-RAY DIFFRACTION / Resolution: 2.533 Å

PDB-6s3o:
Crystal structure of helicase Pif1 from Thermus oshimai in complex with ssDNA (dT)18 and ADP
Method: X-RAY DIFFRACTION / Resolution: 1.974 Å

PDB-6s3p:
Crystal structure of helicase Pif1 from Thermus oshimai in complex with (dT)18
Method: X-RAY DIFFRACTION / Resolution: 1.926 Å

PDB-6xzt:
Crystal structure of helicase Pif1 from Thermus oshimai mutant G110C-E410C
Method: X-RAY DIFFRACTION / Resolution: 3.341 Å

PDB-7ada:
Crystal structure of helicase Pif1 from Thermus oshimai mutant Q164C-E409C
Method: X-RAY DIFFRACTION / Resolution: 3.34 Å

PDB-7bil:
Crystal structure of helicase Pif1 from Thermus oshimai in complex with oligo GGTTTGGTTTGGTT
Method: X-RAY DIFFRACTION / Resolution: 2.21 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-ALF:
TETRAFLUOROALUMINATE ION

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-MF4:
TETRAFLUOROMAGNESATE(2-)

ChemComp-VO4:
VANADATE ION

ChemComp-K:
Unknown entry

Source
  • thermus oshimai jl-2 (bacteria)
  • thermus oshimai (bacteria)
KeywordsHYDROLASE / DNA helicase

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