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Structure paper

TitleStructure of the processive human Pol δ holoenzyme.
Journal, issue, pagesNat Commun, Vol. 11, Issue 1, Page 1109, Year 2020
Publish dateFeb 28, 2020
AuthorsClaudia Lancey / Muhammad Tehseen / Vlad-Stefan Raducanu / Fahad Rashid / Nekane Merino / Timothy J Ragan / Christos G Savva / Manal S Zaher / Afnan Shirbini / Francisco J Blanco / Samir M Hamdan / Alfredo De Biasio /
PubMed AbstractIn eukaryotes, DNA polymerase δ (Pol δ) bound to the proliferating cell nuclear antigen (PCNA) replicates the lagging strand and cooperates with flap endonuclease 1 (FEN1) to process the Okazaki ...In eukaryotes, DNA polymerase δ (Pol δ) bound to the proliferating cell nuclear antigen (PCNA) replicates the lagging strand and cooperates with flap endonuclease 1 (FEN1) to process the Okazaki fragments for their ligation. We present the high-resolution cryo-EM structure of the human processive Pol δ-DNA-PCNA complex in the absence and presence of FEN1. Pol δ is anchored to one of the three PCNA monomers through the C-terminal domain of the catalytic subunit. The catalytic core sits on top of PCNA in an open configuration while the regulatory subunits project laterally. This arrangement allows PCNA to thread and stabilize the DNA exiting the catalytic cleft and recruit FEN1 to one unoccupied monomer in a toolbelt fashion. Alternative holoenzyme conformations reveal important functional interactions that maintain PCNA orientation during synthesis. This work sheds light on the structural basis of Pol δ's activity in replicating the human genome.
External linksNat Commun / PubMed:32111820 / PubMed Central
MethodsEM (single particle)
Resolution3.08 - 8.1 Å
Structure data

10080

6s1m

EMDB-10080, PDB-6s1m:
Human polymerase delta holoenzyme Conformer 1
Method: EM (single particle) / Resolution: 4.27 Å

10081

6s1n

EMDB-10081, PDB-6s1n:
Human polymerase delta holoenzyme Conformer 2
Method: EM (single particle) / Resolution: 4.86 Å

10082

6s1o

EMDB-10082, PDB-6s1o:
Human polymerase delta holoenzyme Conformer 3
Method: EM (single particle) / Resolution: 8.1 Å

10539

6tny

EMDB-10539, PDB-6tny:
Processive human polymerase delta holoenzyme
Method: EM (single particle) / Resolution: 3.08 Å

10540

6tnz

EMDB-10540, PDB-6tnz:
Human polymerase delta-FEN1-PCNA toolbelt
Method: EM (single particle) / Resolution: 4.05 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-TTP:
THYMIDINE-5'-TRIPHOSPHATE

Source
  • homo sapiens (human)
  • synthetic construct (others)
KeywordsREPLICATION / Protein

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