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Structure paper

TitleA mechanism to prevent production of reactive oxygen species by Escherichia coli respiratory complex I.
Journal, issue, pagesNat Commun, Vol. 10, Page 2551-2551, Year 2019
Publish dateSep 10, 2018 (structure data deposition date)
AuthorsSchulte, M. / Frick, K. / Gnandt, E. / Jurkovic, S. / Burschel, S. / Labatzke, R. / Aierstock, K. / Fiegen, D. / Wohlwend, D. / Gerhardt, S. ...Schulte, M. / Frick, K. / Gnandt, E. / Jurkovic, S. / Burschel, S. / Labatzke, R. / Aierstock, K. / Fiegen, D. / Wohlwend, D. / Gerhardt, S. / Einsle, O. / Friedrich, T.
External linksNat Commun / PubMed:31186428
MethodsX-ray diffraction
Resolution1.78 - 3.22 Å
Structure data

PDB-6hl2:
wild-type NuoEF from Aquifex aeolicus - oxidized form
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

PDB-6hl3:
wild-type NuoEF from Aquifex aeolicus - oxidized form bound to NAD+
Method: X-RAY DIFFRACTION / Resolution: 2.04 Å

PDB-6hl4:
wild-type NuoEF from Aquifex aeolicus - reduced form
Method: X-RAY DIFFRACTION / Resolution: 2.06 Å

PDB-6hla:
wild-type NuoEF from Aquifex aeolicus - reduced form bound to NADH
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-6hli:
wild-type NuoEF from Aquifex aeolicus - reduced form bound to NAD+
Method: X-RAY DIFFRACTION / Resolution: 2.38 Å

PDB-6hlj:
Variant G129S of NuoEF from Aquifex aeolicus - oxidized from
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-6hlm:
Variant G129D of NuoEF from Aquifex aeolicus bound to NAD+
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-6q9c:
Crystal structure of Aquifex aeolicus NADH-quinone oxidoreductase subunits NuoE and NuoF bound to NADH under anaerobic conditions
Method: X-RAY DIFFRACTION / Resolution: 1.78 Å

PDB-6q9g:
Crystal structure of reduced Aquifex aeolicus NADH-quinone oxidoreductase subunits NuoE G129D and NuoF bound to NADH
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-6q9j:
Crystal structure of reduced Aquifex aeolicus NADH-quinone oxidoreductase subunits NuoE G129S and NuoF bound to NADH
Method: X-RAY DIFFRACTION / Resolution: 1.83 Å

PDB-6q9k:
Crystal structure of reduced Aquifex aeolicus NADH-quinone oxidoreductase subunits NuoE and NuoF S96M bound to NADH
Method: X-RAY DIFFRACTION / Resolution: 1.99 Å

PDB-6r7p:
Crystal structure of oxidized Aquifex aeolicus NADH-quinone oxidoreductase subunits NuoE and NuoF S96M
Method: X-RAY DIFFRACTION / Resolution: 3.22 Å

Chemicals

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER / Iron–sulfur cluster

ChemComp-SO4:
SULFATE ION / Sulfate

ChemComp-CL:
Unknown entry / Chloride

ChemComp-SF4:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE / Flavin mononucleotide

ChemComp-NA:
Unknown entry

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM / Tris

ChemComp-HOH:
WATER / Water

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM / Nicotinamide adenine dinucleotide

ChemComp-MPO:
3[N-MORPHOLINO]PROPANE SULFONIC ACID / pH buffer*YM / MOPS

ChemComp-NAI:
1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE / Nicotinamide adenine dinucleotide

ChemComp-GOL:
GLYCEROL / Glycerol

Source
  • aquifex aeolicus (bacteria)
  • aquifex aeolicus vf5 (bacteria)
  • aquifex aeolicus (strain vf5) (bacteria)
KeywordsELECTRON TRANSPORT / Complex I / NuoEF / electron transfer / Aquifex aeolicus / OXIDOREDUCTASE / respiratory chain / NADH ubiquinone oxidoreductase / Fe-S clusters

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