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Title | Cryo-EM structure of the rhodopsin-Gαi-βγ complex reveals binding of the rhodopsin C-terminal tail to the gβ subunit. |
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Journal, issue, pages | Elife, Vol. 8, Year 2019 |
Publish date | Jun 28, 2019 |
![]() | Ching-Ju Tsai / Jacopo Marino / Ricardo Adaixo / Filip Pamula / Jonas Muehle / Shoji Maeda / Tilman Flock / Nicholas Mi Taylor / Inayatulla Mohammed / Hugues Matile / Roger Jp Dawson / Xavier Deupi / Henning Stahlberg / Gebhard Schertler / ![]() |
PubMed Abstract | One of the largest membrane protein families in eukaryotes are G protein-coupled receptors (GPCRs). GPCRs modulate cell physiology by activating diverse intracellular transducers, prominently ...One of the largest membrane protein families in eukaryotes are G protein-coupled receptors (GPCRs). GPCRs modulate cell physiology by activating diverse intracellular transducers, prominently heterotrimeric G proteins. The recent surge in structural data has expanded our understanding of GPCR-mediated signal transduction. However, many aspects, including the existence of transient interactions, remain elusive. We present the cryo-EM structure of the light-sensitive GPCR rhodopsin in complex with heterotrimeric Gi. Our density map reveals the receptor C-terminal tail bound to the Gβ subunit of the G protein, providing a structural foundation for the role of the C-terminal tail in GPCR signaling, and of Gβ as scaffold for recruiting Gα subunits and G protein-receptor kinases. By comparing available complexes, we found a small set of common anchoring points that are G protein-subtype specific. Taken together, our structure and analysis provide new structural basis for the molecular events of the GPCR signaling pathway. |
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Methods | EM (single particle) / X-ray diffraction |
Resolution | 1.9 - 4.38 Å |
Structure data | ![]() PDB-6qnk: |
Chemicals | ![]() ChemComp-PG4: ![]() ChemComp-EDO: ![]() ChemComp-PGE: ![]() ChemComp-MLT: ![]() ChemComp-HOH: ![]() ChemComp-RET: |
Source |
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![]() | IMMUNE SYSTEM / Antibody FAB fragment / SIGNALING PROTEIN / GPCR and G protein complex |