Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM.
Journal, issue, pages	Nat Commun, Vol. 10, Issue 1, Page 1722, Year 2019
Publish date	Apr 12, 2019
Authors	Ricardo D Righetto / Nikhil Biyani / Julia Kowal / Mohamed Chami / Henning Stahlberg /
PubMed Abstract	Electron crystallography can reveal the structure of membrane proteins within 2D crystals under close-to-native conditions. High-resolution structural information can only be reached if crystals are ...Electron crystallography can reveal the structure of membrane proteins within 2D crystals under close-to-native conditions. High-resolution structural information can only be reached if crystals are perfectly flat and highly ordered. In practice, such crystals are difficult to obtain. Available image unbending algorithms correct for disorder, but only perform well on images of non-tilted, flat crystals, while out-of-plane distortions are not addressed. Here, we present an approach that employs single-particle refinement procedures to locally unbend crystals in 3D. With this method, density maps of the MloK1 potassium channel with a resolution of 4 Å were obtained from images of 2D crystals that do not diffract beyond 10 Å. Furthermore, 3D classification allowed multiple structures to be resolved, revealing a series of MloK1 conformations within a single 2D crystal. This conformational heterogeneity explains the poor diffraction observed and is related to channel function. The approach is implemented in the FOCUS package.
External links	Nat Commun / PubMed:30979902 / PubMed Central
Methods	EM (single particle)
Resolution	4.0 - 6.0 Å
Structure data	4432 6i9d EMDB-4432: MloK1 consensus map from single particle analysis of 2D crystals PDB-6i9d: MloK1 consensus structure from single particle analysis of 2D crystals Method: EM (single particle) / Resolution: 4.0 Å EMDB-4439: Full MloK1 consensus map from single particle analysis of 2D crystals Method: EM (single particle) / Resolution: 6.0 Å 4441 6iax EMDB-4441: MloK1 map from single particle analysis of 2D crystals, class 1 (extended conformation) PDB-6iax: MloK1 model from single particle analysis of 2D crystals, class 1 (extended conformation) Method: EM (single particle) / Resolution: 5.2 Å 4513 6qcy EMDB-4513: MloK1 map from single particle analysis of 2D crystals, class 2 (intermediate conformation) PDB-6qcy: MloK1 model from single particle analysis of 2D crystals, class 2 (intermediate conformation) Method: EM (single particle) / Resolution: 4.7 Å 4514 6qcz EMDB-4514: MloK1 map from single particle analysis of 2D crystals, class 3 (intermediate extended conformation) PDB-6qcz: MloK1 model from single particle analysis of 2D crystals, class 3 (intermediate extended conformation) Method: EM (single particle) / Resolution: 4.4 Å 4515 6qd0 EMDB-4515: MloK1 map from single particle analysis of 2D crystals, class 4 (compact/open conformation) PDB-6qd0: MloK1 model from single particle analysis of 2D crystals, class 4 (compact/open conformation) Method: EM (single particle) / Resolution: 4.5 Å 4516 6qd1 EMDB-4516: MloK1 map from single particle analysis of 2D crystals, class 5 (intermediate compact conformation) PDB-6qd1: MloK1 model from single particle analysis of 2D crystals, class 5 (intermediate compact conformation) Method: EM (single particle) / Resolution: 5.4 Å 4517 6qd2 EMDB-4517: MloK1 map from single particle analysis of 2D crystals, class 6 (intermediate compact conformation) PDB-6qd2: MloK1 model from single particle analysis of 2D crystals, class 6 (intermediate compact conformation) Method: EM (single particle) / Resolution: 4.8 Å 4518 6qd3 EMDB-4518: MloK1 map from single particle analysis of 2D crystals, class 7 (intermediate conformation) PDB-6qd3: MloK1 model from single particle analysis of 2D crystals, class 7 (intermediate conformation) Method: EM (single particle) / Resolution: 5.0 Å 4519 6qd4 EMDB-4519, PDB-6qd4: MloK1 model from single particle analysis of 2D crystals, class 8 (intermediate conformation) Method: EM (single particle) / Resolution: 5.6 Å
Chemicals	ChemComp-K: Unknown entry
Source	mesorhizobium loti maff303099 (bacteria) mesorhizobium japonicum (strain lmg 29417 / cect 9101 / maff 303099) (bacteria)
Keywords	MEMBRANE PROTEIN / Voltage-gated potassium channel / Cyclic nucleotide-binding domain / ion channel / ion transport