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Structure paper

TitleToward a Structural Understanding of Class B GPCR Peptide Binding and Activation.
Journal, issue, pagesMol Cell, Vol. 77, Issue 3, Page 656-668.e5, Year 2020
Publish dateFeb 6, 2020
AuthorsYi-Lynn Liang / Matthew J Belousoff / Peishen Zhao / Cassandra Koole / Madeleine M Fletcher / Tin T Truong / Villy Julita / George Christopoulos / H Eric Xu / Yan Zhang / Maryam Khoshouei / Arthur Christopoulos / Radostin Danev / Patrick M Sexton / Denise Wootten /
PubMed AbstractClass B G protein-coupled receptors (GPCRs) are important therapeutic targets for major diseases. Here, we present structures of peptide and Gs-bound pituitary adenylate cyclase-activating peptide, ...Class B G protein-coupled receptors (GPCRs) are important therapeutic targets for major diseases. Here, we present structures of peptide and Gs-bound pituitary adenylate cyclase-activating peptide, PAC1 receptor, and corticotropin-releasing factor (CRF), (CRF1) receptor. Together with recently solved structures, these provide coverage of the major class B GPCR subfamilies. Diverse orientations of the extracellular domain to the receptor core in different receptors are at least partially dependent on evolutionary conservation in the structure and nature of peptide interactions. Differences in peptide interactions to the receptor core also influence the interlinked TM2-TM1-TM6/ECL3/TM7 domain, and this is likely important in their diverse signaling. However, common conformational reorganization of ECL2, linked to reorganization of ICL2, modulates G protein contacts. Comparison between receptors reveals ICL2 as a key domain forming dynamic G protein interactions in a receptor- and ligand-specific manner. This work advances our understanding of class B GPCR activation and Gs coupling.
External linksMol Cell / PubMed:32004469
MethodsEM (single particle)
Resolution2.65 - 3.01 Å
Structure data

EMDB-0993:
Cryo-EM map of PAC1 receptor bound to PACAP38 and Gs protein
Method: EM (single particle) / Resolution: 2.65 Å

20277

6p9x

EMDB-20277, PDB-6p9x:
CRF1 Receptor Gs GPCR protein complex with CRF1 peptide
Method: EM (single particle) / Resolution: 2.91 Å

20278

6p9y

EMDB-20278, PDB-6p9y:
PAC1 GPCR Receptor complex
Method: EM (single particle) / Resolution: 3.01 Å

EMDB-30003:
Cryo-EM map of CRF1 receptor bound to CRF and Gs protein
Method: EM (single particle) / Resolution: 2.7 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • homo sapiens (human)
  • lama glama (llama)
KeywordsMEMBRANE PROTEIN / GPCR

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