Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structural basis for selective stalling of human ribosome nascent chain complexes by a drug-like molecule.
Journal, issue, pages	Nat Struct Mol Biol, Vol. 26, Issue 6, Page 501-509, Year 2019
Publish date	Jun 3, 2019
Authors	Wenfei Li / Fred R Ward / Kim F McClure / Stacey Tsai-Lan Chang / Elizabeth Montabana / Spiros Liras / Robert G Dullea / Jamie H D Cate /
PubMed Abstract	The drug-like molecule PF-06446846 (PF846) binds the human ribosome and selectively blocks the translation of a small number of proteins by an unknown mechanism. In structures of PF846-stalled human ...The drug-like molecule PF-06446846 (PF846) binds the human ribosome and selectively blocks the translation of a small number of proteins by an unknown mechanism. In structures of PF846-stalled human ribosome nascent chain complexes, PF846 binds in the ribosome exit tunnel in a eukaryotic-specific pocket formed by 28S ribosomal RNA, and alters the path of the nascent polypeptide chain. PF846 arrests the translating ribosome in the rotated state of translocation, in which the peptidyl-transfer RNA 3'-CCA end is improperly docked in the peptidyl transferase center. Selections of messenger RNAs from mRNA libraries using translation extracts reveal that PF846 can stall translation elongation, arrest termination or even enhance translation, depending on nascent chain sequence context. These results illuminate how a small molecule selectively targets translation by the human ribosome, and provides a foundation for developing small molecules that modulate the production of proteins of therapeutic interest.
External links	Nat Struct Mol Biol / PubMed:31160784 / PubMed Central
Methods	EM (single particle)
Resolution	3.0 - 4.7 Å
Structure data	0526 6oli EMDB-0526: Structure of drug-like molecule stalled USO1 ribosome nascent chain complex (human) PDB-6oli: Structure of human ribosome nascent chain complex selectively stalled by a drug-like small molecule (USO1-RNC) Method: EM (single particle) / Resolution: 3.5 Å 0596 6om0 EMDB-0596: Structure of a drug-like molecule stalled PCSK9 ribosome nascent chain complex (PCSK9-RNC) with AP tRNA and PE tRNA PDB-6om0: Human ribosome nascent chain complex (PCSK9-RNC) stalled by a drug-like molecule with AP and PE tRNAs Method: EM (single particle) / Resolution: 3.08 Å 0597 6om7 EMDB-0597: Structure of a drug-like molecule stalled ribosome nascent chain complex (PCSK9-RNC) in rotated state with AA tRAN and PE tRNA PDB-6om7: Human ribosome nascent chain complex (PCSK9-RNC) stalled by a drug-like small molecule with AA and PE tRNAs Method: EM (single particle) / Resolution: 3.72 Å 0598 6olz EMDB-0598: Structure of a drug-like molecule stalled ribosome nascent chain complex (PCSK9_RNC) in non-rotated state with PP tRNA PDB-6olz: Human ribosome nascent chain complex (PCSK9-RNC) stalled by a drug-like molecule with PP tRNA Method: EM (single particle) / Resolution: 3.9 Å 0599 6olf EMDB-0599: Structure of a drug-like molecule stalled CDH1 ribosome nascent chain complex (CDH1-RNC) in rotated state with AA tRNA and PE tRNA PDB-6olf: Human ribosome nascent chain complex (CDH1-RNC) stalled by a drug-like molecule with AA and PE tRNAs Method: EM (single particle) / Resolution: 3.9 Å 0600 6ole EMDB-0600: Human ribosome nascent chain complex (CDH1-RNC) stalled by a drug-like molecule with AP tRNA and PE tRNA PDB-6ole: Human ribosome nascent chain complex (CDH1-RNC) stalled by a drug-like molecule with AP and PE tRNAs Method: EM (single particle) / Resolution: 3.0 Å 0601 6olg EMDB-0601: Human ribosome nascent chain complex (CDH1-RNC) stalled by a drug-like molecule with PP tRNA PDB-6olg: Human ribosome nascent chain complex stalled by a drug-like small molecule (CDH1_RNC with PP tRNA) Method: EM (single particle) / Resolution: 3.4 Å EMDB-20134: Structure of a drug-like molecule stalled PCSK9 ribosome nascent chain complex (PCSK9-RNC) with AP tRNA and PE tRNA (sample prepared with a short incubation time) Method: EM (single particle) / Resolution: 4.7 Å EMDB-20135: Structure of a drug-like molecule stalled PCSK9 ribosome nascent chain complex (PCSK9-RNC) with PP tRNA (sample prepared with a short incubation time) Method: EM (single particle) / Resolution: 4.6 Å
Chemicals	ChemComp-MG: Unknown entry ChemComp-ZN: Unknown entry ChemComp-MVM: N-(3-chloropyridin-2-yl)-N-[(3R)-piperidin-3-yl]-4-(3H-[1,2,3]triazolo[4,5-b]pyridin-3-yl)benzamide ChemComp-HOH: WATER
Source	homo sapiens (human)
Keywords	RIBOSOME / selective stalling / drug-like molecule / human ribosome nascent chain complex / drug-like small molecule