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TitleThe structure of a 15-stranded actin-like filament from Clostridium botulinum.
Journal, issue, pagesNat Commun, Vol. 10, Issue 1, Page 2856, Year 2019
Publish dateJun 28, 2019
AuthorsFujiet Koh / Akihiro Narita / Lin Jie Lee / Kotaro Tanaka / Yong Zi Tan / Venkata P Dandey / David Popp / Robert C Robinson /
PubMed AbstractMicrofilaments (actin) and microtubules represent the extremes in eukaryotic cytoskeleton cross-sectional dimensions, raising the question of whether filament architectures are limited by protein ...Microfilaments (actin) and microtubules represent the extremes in eukaryotic cytoskeleton cross-sectional dimensions, raising the question of whether filament architectures are limited by protein fold. Here, we report the cryoelectron microscopy structure of a complex filament formed from 15 protofilaments of an actin-like protein. This actin-like ParM is encoded on the large pCBH Clostridium botulinum plasmid. In cross-section, the ~26 nm diameter filament comprises a central helical protofilament surrounded by intermediate and outer layers of six and eight twisted protofilaments, respectively. Alternating polarity of the layers allows for similar lateral contacts between each layer. This filament design is stiffer than the actin filament, and has likely been selected for during evolution to move large cargos. The comparable sizes of microtubule and pCBH ParM filaments indicate that larger filament architectures are not limited by the protomer fold. Instead, function appears to have been the evolutionary driving force to produce broad, complex filaments.
External linksNat Commun / PubMed:31253774 / PubMed Central
MethodsEM (helical sym.) / X-ray diffraction
Resolution3.253 - 4.7 Å
Structure data

9757

6izr

EMDB-9757: pCBH ParM filament
PDB-6izr: Whole structure of a 15-stranded ParM filament from Clostridium botulinum
Method: EM (helical sym.) / Resolution: 4.7 Å

9758

6izv

EMDB-9758: pCBH ParM filament
PDB-6izv: Averaged strand structure of a 15-stranded ParM filament from Clostridium botulinum
Method: EM (helical sym.) / Resolution: 4.2 Å

PDB-6ixw:
pCBH ParM non-polymerisable quadruple mutant
Method: X-RAY DIFFRACTION / Resolution: 3.253 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

Source
  • Clostridium botulinum F str. 230613 (bacteria)
  • clostridium botulinum prevot_594 (bacteria)
KeywordsSTRUCTURAL PROTEIN / Actin ParM / PROTEIN FIBRIL / ParM / filaments / cytoskeleton

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