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TitleMitotic phosphorylation by NEK6 and NEK7 reduces the microtubule affinity of EML4 to promote chromosome congression.
Journal, issue, pagesSci Signal, Vol. 12, Issue 594, Year 2019
Publish dateAug 13, 2019
AuthorsRozita Adib / Jessica M Montgomery / Joseph Atherton / Laura O'Regan / Mark W Richards / Kees R Straatman / Daniel Roth / Anne Straube / Richard Bayliss / Carolyn A Moores / Andrew M Fry /
PubMed AbstractEML4 is a microtubule-associated protein that promotes microtubule stability. We investigated its regulation across the cell cycle and found that EML4 was distributed as punctate foci along the ...EML4 is a microtubule-associated protein that promotes microtubule stability. We investigated its regulation across the cell cycle and found that EML4 was distributed as punctate foci along the microtubule lattice in interphase but exhibited reduced association with spindle microtubules in mitosis. Microtubule sedimentation and cryo-electron microscopy with 3D reconstruction revealed that the basic N-terminal domain of EML4 mediated its binding to the acidic C-terminal tails of α- and β-tubulin on the microtubule surface. The mitotic kinases NEK6 and NEK7 phosphorylated the EML4 N-terminal domain at Ser and Ser in vitro, and depletion of these kinases in cells led to increased EML4 binding to microtubules in mitosis. An S144A-S146A double mutant not only bound inappropriately to mitotic microtubules but also increased their stability and interfered with chromosome congression. In addition, constitutive activation of NEK6 or NEK7 reduced the association of EML4 with interphase microtubules. Together, these data support a model in which NEK6- and NEK7-dependent phosphorylation promotes the dissociation of EML4 from microtubules in mitosis in a manner that is required for efficient chromosome congression.
External linksSci Signal / PubMed:31409757
MethodsEM (single particle)
Resolution3.6 Å
Structure data

0331

6i2i

EMDB-0331, PDB-6i2i:
Refined 13pf Hela Cell Tubulin microtubule (EML4-NTD decorated)
Method: EM (single particle) / Resolution: 3.6 Å

Chemicals

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-G2P:
PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMP-CPP, energy-carrying molecule analogue*YM

ChemComp-TA1:
TAXOL / medication, chemotherapy*YM

Source
  • homo sapiens (human)
KeywordsSTRUCTURAL PROTEIN / Microtubule / Tubulin / Hela / EML

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