EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural transitions of F-actin upon ATP hydrolysis at near-atomic resolution revealed by cryo-EM.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 25, Issue 6, Page 528-537, Year 2018
掲載日	2018年6月4日
著者	Felipe Merino / Sabrina Pospich / Johanna Funk / Thorsten Wagner / Florian Küllmer / Hans-Dieter Arndt / Peter Bieling / Stefan Raunser /
PubMed 要旨	The function of actin is coupled to the nucleotide bound to its active site. ATP hydrolysis is activated during polymerization; a delay between hydrolysis and inorganic phosphate (P) release results ...The function of actin is coupled to the nucleotide bound to its active site. ATP hydrolysis is activated during polymerization; a delay between hydrolysis and inorganic phosphate (P) release results in a gradient of ATP, ADP-P and ADP along actin filaments (F-actin). Actin-binding proteins can recognize F-actin's nucleotide state, using it as a local 'age' tag. The underlying mechanism is complex and poorly understood. Here we report six high-resolution cryo-EM structures of F-actin from rabbit skeletal muscle in different nucleotide states. The structures reveal that actin polymerization repositions the proposed catalytic base, His161, closer to the γ-phosphate. Nucleotide hydrolysis and P release modulate the conformational ensemble at the periphery of the filament, thus resulting in open and closed states, which can be sensed by coronin-1B. The drug-like toxin jasplakinolide locks F-actin in an open state. Our results demonstrate in detail how ATP hydrolysis links to F-actin's conformational dynamics and protein interaction.
リンク	Nat Struct Mol Biol / PubMed:29867215
手法	EM (単粒子)
解像度	3.3 - 4.1 Å
構造データ	3835 5onv EMDB-3835, PDB-5onv: Cryo-EM structure of F-actin in complex with ADP 手法: EM (単粒子) / 解像度: 4.1 Å 3836 5ooc EMDB-3836, PDB-5ooc: Cryo-EM structure of jasplakinolide-stabilized F-actin in complex with ADP 手法: EM (単粒子) / 解像度: 3.6 Å 3837 5ood EMDB-3837, PDB-5ood: Cryo-EM structure of jasplakinolide-stabilized F-actin in complex with ADP-Pi 手法: EM (単粒子) / 解像度: 3.7 Å 3838 5ooe EMDB-3838, PDB-5ooe: Cryo-EM structure of F-actin in complex with AppNHp (AMPPNP) 手法: EM (単粒子) / 解像度: 3.6 Å 3839 5oof EMDB-3839, PDB-5oof: Cryo-EM structure of F-actin in complex with ADP-BeFx 手法: EM (単粒子) / 解像度: 3.4 Å 4259 6fhl EMDB-4259, PDB-6fhl: Cryo-EM structure of F-actin in complex with ADP-Pi 手法: EM (単粒子) / 解像度: 3.3 Å
化合物	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-9ZK: (4~{R},7~{R},10~{S},13~{S},15~{E},19~{S})-10-(4-azanylbutyl)-4-(4-hydroxyphenyl)-7-(1~{H}-indol-3-ylmethyl)-8,13,15,19-tetramethyl-1-oxa-5,8,11-triazacyclononadec-15-ene-2,6,9,12-tetrone ChemComp-PO4: PHOSPHATE ION / ホスファ-ト / リン酸塩 ChemComp-ANP: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP / AMP-PNP, エネルギー貯蔵分子類似体YM
由来	oryctolagus cuniculus (ウサギ) Rabbit (ウサギ)
キーワード	STRUCTURAL PROTEIN (タンパク質) / Cytoskeleton (細胞骨格) / nucleotide states / filament stability / cell migration (遊走)