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TitleCryo-EM analysis of the T3S injectisome reveals the structure of the needle and open secretin.
Journal, issue, pagesNat Commun, Vol. 9, Issue 1, Page 3840, Year 2018
Publish dateSep 21, 2018
AuthorsJ Hu / L J Worrall / C Hong / M Vuckovic / C E Atkinson / N Caveney / Z Yu / N C J Strynadka /
PubMed AbstractThe bacterial type III secretion system, or injectisome, is a syringe shaped nanomachine essential for the virulence of many disease causing Gram-negative bacteria. At the core of the injectisome ...The bacterial type III secretion system, or injectisome, is a syringe shaped nanomachine essential for the virulence of many disease causing Gram-negative bacteria. At the core of the injectisome structure is the needle complex, a continuous channel formed by the highly oligomerized inner and outer membrane hollow rings and a polymerized helical needle filament which spans through and projects into the infected host cell. Here we present the near-atomic resolution structure of a needle complex from the prototypical Salmonella Typhimurium SPI-1 type III secretion system, with local masking protocols allowing for model building and refinement of the major membrane spanning components of the needle complex base in addition to an isolated needle filament. This work provides significant insight into injectisome structure and assembly and importantly captures the molecular basis for substrate induced gating in the giant outer membrane secretin portal family.
External linksNat Commun / PubMed:30242280 / PubMed Central
MethodsEM (single particle)
Resolution3.3 - 4.1 Å
Structure data

8913

6duz

EMDB-8913, PDB-6duz:
Structure of the periplasmic domains of PrgH and PrgK from the assembled Salmonella type III secretion injectisome needle complex
Method: EM (single particle) / Resolution: 3.6 Å

8914

6dv3

EMDB-8914, PDB-6dv3:
Structure of the Salmonella SPI-1 type III secretion injectisome secretin InvG in the open gate state
Method: EM (single particle) / Resolution: 4.1 Å

8915

6dv6

EMDB-8915, PDB-6dv6:
Structure of the Salmonella SPI-1 type III secretion injectisome secretin InvG (residues 176-end) in the open gate state
Method: EM (single particle) / Resolution: 3.9 Å

8924

6dwb

EMDB-8924, PDB-6dwb:
Structure of the Salmonella SPI-1 type III secretion injectisome needle filament
Method: EM (single particle) / Resolution: 3.3 Å

Source
  • salmonella enterica subsp. enterica serovar typhimurium (bacteria)
KeywordsMEMBRANE PROTEIN / Type III secretion system / secretin

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