EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Analysis of Global and Site-Specific Radiation Damage in Cryo-EM.
ジャーナル・号・ページ	Structure, Vol. 26, Issue 5, Page 759-766.e4, Year 2018
掲載日	2018年5月1日
著者	Johan Hattne / Dan Shi / Calina Glynn / Chih-Te Zee / Marcus Gallagher-Jones / Michael W Martynowycz / Jose A Rodriguez / Tamir Gonen /
PubMed 要旨	Micro-crystal electron diffraction (MicroED) combines the efficiency of electron scattering with diffraction to allow structure determination from nano-sized crystalline samples in cryoelectron ...Micro-crystal electron diffraction (MicroED) combines the efficiency of electron scattering with diffraction to allow structure determination from nano-sized crystalline samples in cryoelectron microscopy (cryo-EM). It has been used to solve structures of a diverse set of biomolecules and materials, in some cases to sub-atomic resolution. However, little is known about the damaging effects of the electron beam on samples during such measurements. We assess global and site-specific damage from electron radiation on nanocrystals of proteinase K and of a prion hepta-peptide and find that the dynamics of electron-induced damage follow well-established trends observed in X-ray crystallography. Metal ions are perturbed, disulfide bonds are broken, and acidic side chains are decarboxylated while the diffracted intensities decay exponentially with increasing exposure. A better understanding of radiation damage in MicroED improves our assessment and processing of all types of cryo-EM data.
リンク	Structure / PubMed:29706530 / PubMed Central
手法	EM (電子線結晶学)
解像度	1.01 - 3.2 Å
構造データ	7490 6cl7 EMDB-7490, PDB-6cl7: 1.71 A MicroED structure of proteinase K at 0.86 e- / A^2 手法: EM (電子線結晶学) / 解像度: 1.71 Å 7491 6cl8 EMDB-7491, PDB-6cl8: 2.00 A MicroED structure of proteinase K at 2.6 e- / A^2 手法: EM (電子線結晶学) / 解像度: 2.0 Å 7492 6cl9 EMDB-7492, PDB-6cl9: 2.20 A MicroED structure of proteinase K at 4.3 e- / A^2 手法: EM (電子線結晶学) / 解像度: 2.2 Å 7493 6cla EMDB-7493, PDB-6cla: 2.80 A MicroED structure of proteinase K at 6.0 e- / A^2 手法: EM (電子線結晶学) / 解像度: 2.8 Å 7494 6clb EMDB-7494, PDB-6clb: 3.20 A MicroED structure of proteinase K at 7.8 e- / A^2 手法: EM (電子線結晶学) / 解像度: 3.2 Å 7495 6clc EMDB-7495, PDB-6clc: 1.01 A MicroED structure of GSNQNNF at 0.27 e- / A^2 手法: EM (電子線結晶学) / 解像度: 1.01 Å 7496 6cld EMDB-7496, PDB-6cld: 1.01 A MicroED structure of GSNQNNF at 0.81 e- / A^2 手法: EM (電子線結晶学) / 解像度: 1.01 Å 7497 6cle EMDB-7497, PDB-6cle: 1.01 A MicroED structure of GSNQNNF at 1.3 e- / A^2 手法: EM (電子線結晶学) / 解像度: 1.01 Å 7498 6clf EMDB-7498, PDB-6clf: 1.15 A MicroED structure of GSNQNNF at 1.9 e- / A^2 手法: EM (電子線結晶学) / 解像度: 1.15 Å 7499 6clg EMDB-7499, PDB-6clg: 1.35 A MicroED structure of GSNQNNF at 2.4 e- / A^2 手法: EM (電子線結晶学) / 解像度: 1.35 Å 7500 6clh EMDB-7500, PDB-6clh: 1.37 A MicroED structure of GSNQNNF at 2.9 e- / A^2 手法: EM (電子線結晶学) / 解像度: 1.37 Å 7501 6cli EMDB-7501, PDB-6cli: 1.01 A MicroED structure of GSNQNNF at 0.17 e- / A^2 手法: EM (電子線結晶学) / 解像度: 1.01 Å 7502 6clj EMDB-7502, PDB-6clj: 1.01 A MicroED structure of GSNQNNF at 0.50 e- / A^2 手法: EM (電子線結晶学) / 解像度: 1.01 Å 7503 6clk EMDB-7503, PDB-6clk: 1.01 A MicroED structure of GSNQNNF at 0.82 e- / A^2 手法: EM (電子線結晶学) / 解像度: 1.01 Å 7504 6cll EMDB-7504, PDB-6cll: 1.02 A MicroED structure of GSNQNNF at 1.2 e- / A^2 手法: EM (電子線結晶学) / 解像度: 1.02 Å 7505 6clm EMDB-7505, PDB-6clm: 1.01 A MicroED structure of GSNQNNF at 1.5 e- / A^2 手法: EM (電子線結晶学) / 解像度: 1.01 Å 7506 6cln EMDB-7506, PDB-6cln: 1.15 A MicroED structure of GSNQNNF at 1.8 e- / A^2 手法: EM (電子線結晶学) / 解像度: 1.15 Å 7507 6clo EMDB-7507, PDB-6clo: 1.15 A MicroED structure of GSNQNNF at 2.1 e- / A^2 手法: EM (電子線結晶学) / 解像度: 1.15 Å 7508 6clp EMDB-7508, PDB-6clp: 1.16 A MicroED structure of GSNQNNF at 2.5 e- / A^2 手法: EM (電子線結晶学) / 解像度: 1.16 Å 7509 6clq EMDB-7509, PDB-6clq: 1.21 A MicroED structure of GSNQNNF at 2.8 e- / A^2 手法: EM (電子線結晶学) / 解像度: 1.21 Å 7510 6clr EMDB-7510, PDB-6clr: 1.31 A MicroED structure of GSNQNNF at 3.1 e- / A^2 手法: EM (電子線結晶学) / 解像度: 1.31 Å 7511 6cls EMDB-7511, PDB-6cls: 1.46 A MicroED structure of GSNQNNF at 3.4 e- / A^2 手法: EM (電子線結晶学) / 解像度: 1.46 Å 7512 6clt EMDB-7512, PDB-6clt: 1.45 A MicroED structure of GSNQNNF at 3.8 e- / A^2 手法: EM (電子線結晶学) / 解像度: 1.45 Å
化合物	ChemComp-ACT: ACETATE ION / 酢酸イオン ChemComp-ZN: Unknown entry ChemComp-HOH: WATER
由来	Engyodontium album (菌類) parengyodontium album (菌類) synthetic construct (人工物)
キーワード	HYDROLASE / PROTEIN FIBRIL / Amyloid fibril / prion / zinc binding