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TitleStructure and mechanogating mechanism of the Piezo1 channel.
Journal, issue, pagesNature, Vol. 554, Issue 7693, Page 487-492, Year 2018
Publish dateFeb 22, 2018
AuthorsQiancheng Zhao / Heng Zhou / Shaopeng Chi / Yanfeng Wang / Jianhua Wang / Jie Geng / Kun Wu / Wenhao Liu / Tingxin Zhang / Meng-Qiu Dong / Jiawei Wang / Xueming Li / Bailong Xiao /
PubMed AbstractThe mechanosensitive Piezo channels function as key eukaryotic mechanotransducers. However, their structures and mechanogating mechanisms remain unknown. Here we determine the three-bladed, propeller- ...The mechanosensitive Piezo channels function as key eukaryotic mechanotransducers. However, their structures and mechanogating mechanisms remain unknown. Here we determine the three-bladed, propeller-like electron cryo-microscopy structure of mouse Piezo1 and functionally reveal its mechanotransduction components. Despite the lack of sequence repetition, we identify nine repetitive units consisting of four transmembrane helices each-which we term transmembrane helical units (THUs)-which assemble into a highly curved blade-like structure. The last transmembrane helix encloses a hydrophobic pore, followed by three intracellular fenestration sites and side portals that contain pore-property-determining residues. The central region forms a 90 Å-long intracellular beam-like structure, which undergoes a lever-like motion to connect THUs to the pore via the interfaces of the C-terminal domain, the anchor-resembling domain and the outer helix. Deleting extracellular loops in the distal THUs or mutating single residues in the beam impairs the mechanical activation of Piezo1. Overall, Piezo1 possesses a unique 38-transmembrane-helix topology and designated mechanotransduction components, which enable a lever-like mechanogating mechanism.
External linksNature / PubMed:29469092
MethodsEM (single particle)
Resolution3.97 Å
Structure data

6865

5z10

EMDB-6865, PDB-5z10:
Structure of the mechanosensitive Piezo1 channel
Method: EM (single particle) / Resolution: 3.97 Å

Source
  • mus musculus (house mouse)
KeywordsMEMBRANE PROTEIN / Piezo1 / Piezo2 / mechanogating / channel

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