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TitleImprovement of ST0452N-Acetylglucosamine-1-Phosphate Uridyltransferase Activity by the Cooperative Effect of Two Single Mutations Identified through Structure-Based Protein Engineering
Journal, issue, pagesAppl. Environ. Microbiol., Vol. 84, Year 2018
Publish dateDec 19, 2017 (structure data deposition date)
AuthorsHonda, Y. / Nakano, S. / Ito, S. / Dadashipour, M. / Zhang, Z. / Kawarabayasi, Y.
External linksAppl. Environ. Microbiol. / PubMed:30291121
MethodsX-ray diffraction
Resolution2.09 - 2.91 Å
Structure data

PDB-5z09:
ST0452(Y97N)-UTP binding form
Method: X-RAY DIFFRACTION / Resolution: 2.91 Å

PDB-5z0a:
ST0452(Y97N)-GlcNAc binding form
Method: X-RAY DIFFRACTION / Resolution: 2.09 Å

Chemicals

ChemComp-UTP:
URIDINE 5'-TRIPHOSPHATE / UTP*YM

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-HOH:
WATER

Source
  • sulfolobus tokodaii (strain dsm 16993 / jcm 10545 / nbrc 100140 / 7) (archaea)
KeywordsSUGAR BINDING PROTEIN / UTP binding / ST0452 / GlcNAc binding form

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