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TitleCryo-EM structures of MERS-CoV and SARS-CoV spike glycoproteins reveal the dynamic receptor binding domains.
Journal, issue, pagesNat Commun, Vol. 8, Page 15092, Year 2017
Publish dateApr 10, 2017
AuthorsYuan Yuan / Duanfang Cao / Yanfang Zhang / Jun Ma / Jianxun Qi / Qihui Wang / Guangwen Lu / Ying Wu / Jinghua Yan / Yi Shi / Xinzheng Zhang / George F Gao /
PubMed AbstractThe envelope spike (S) proteins of MERS-CoV and SARS-CoV determine the virus host tropism and entry into host cells, and constitute a promising target for the development of prophylactics and ...The envelope spike (S) proteins of MERS-CoV and SARS-CoV determine the virus host tropism and entry into host cells, and constitute a promising target for the development of prophylactics and therapeutics. Here, we present high-resolution structures of the trimeric MERS-CoV and SARS-CoV S proteins in its pre-fusion conformation by single particle cryo-electron microscopy. The overall structures resemble that from other coronaviruses including HKU1, MHV and NL63 reported recently, with the exception of the receptor binding domain (RBD). We captured two states of the RBD with receptor binding region either buried (lying state) or exposed (standing state), demonstrating an inherently flexible RBD readily recognized by the receptor. Further sequence conservation analysis of six human-infecting coronaviruses revealed that the fusion peptide, HR1 region and the central helix are potential targets for eliciting broadly neutralizing antibodies.
External linksNat Commun / PubMed:28393837 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution1.5 - 4.2 Å
Structure data

6703

5x58

EMDB-6703, PDB-5x58:
Prefusion structure of SARS-CoV spike glycoprotein, conformation 1
Method: EM (single particle) / Resolution: 3.2 Å

6704

5x59

EMDB-6704, PDB-5x59:
Prefusion structure of MERS-CoV spike glycoprotein, three-fold symmetry
Method: EM (single particle) / Resolution: 3.7 Å

6705

5x5b

EMDB-6705, PDB-5x5b:
Prefusion structure of SARS-CoV spike glycoprotein, conformation 2
Method: EM (single particle) / Resolution: 3.7 Å

6706

5x5c

EMDB-6706, PDB-5x5c:
Prefusion structure of MERS-CoV spike glycoprotein, conformation 1
Method: EM (single particle) / Resolution: 4.1 Å

6707

5x5f

EMDB-6707, PDB-5x5f:
Prefusion structure of MERS-CoV spike glycoprotein, conformation 2
Method: EM (single particle) / Resolution: 4.2 Å

PDB-5x4r:
Structure of the N-terminal domain (NTD) of MERS-CoV spike protein
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-5x4s:
Structure of the N-terminal domain (NTD)of SARS-CoV spike protein
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

Source
  • sars coronavirus bj01
  • middle east respiratory syndrome coronavirus
  • human sars coronavirus
KeywordsVIRAL PROTEIN / MERS-CoV / spike / N-terminal domain / SARS-CoV / spike glycoprotein / prefusion / single particle

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