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TitleCryo-EM structure of the extended type VI secretion system sheath-tube complex.
Journal, issue, pagesNat Microbiol, Vol. 2, Issue 11, Page 1507-1512, Year 2017
Publish dateSep 25, 2017
AuthorsJing Wang / Maximilian Brackmann / Daniel Castaño-Díez / Mikhail Kudryashev / Kenneth N Goldie / Timm Maier / Henning Stahlberg / Marek Basler /
PubMed AbstractThe bacterial type VI secretion system (T6SS) uses contraction of a long sheath to quickly thrust a tube with associated effectors across membranes of eukaryotic and bacterial cells . Only limited ...The bacterial type VI secretion system (T6SS) uses contraction of a long sheath to quickly thrust a tube with associated effectors across membranes of eukaryotic and bacterial cells . Only limited structural information is available about the inherently unstable precontraction state of the T6SS. Here, we obtain a 3.7 Å resolution structure of a non-contractile sheath-tube complex using cryo-electron microscopy and show that it resembles the extended T6SS inside Vibrio cholerae cells. We build a pseudo-atomic model of the complete sheath-tube assembly, which provides a mechanistic understanding of coupling sheath contraction with pushing and rotating the inner tube for efficient target membrane penetration. Our data further show that sheath contraction exposes a buried recognition domain to specifically trigger the disassembly and recycling of the T6SS sheath by the cognate ATP-dependent unfoldase ClpV.
External linksNat Microbiol / PubMed:28947741
MethodsEM (subtomogram averaging) / EM (helical sym.)
Resolution3.7 - 17.4 Å
Structure data

EMDB-3563:
Subtomogram average of type VI secretion system (T6SS) extended sheath
Method: EM (subtomogram averaging) / Resolution: 15.9 Å

EMDB-3564:
Subtomogram average of type VI secretion system (T6SS) contracted sheath
Method: EM (subtomogram averaging) / Resolution: 17.4 Å

3566

5mxn

5ojq

EMDB-3566: VipA-N3, non-contractile sheath of the type VI secretion system
PDB-5mxn: Atomic model of the VipA/VipB/Hcp, the type six secretion system non-contractile sheath-tube of Vibrio cholerae from cryo-EM
PDB-5ojq: The modeled structure of of wild type extended type VI secretion system sheath/tube complex in vibrio cholerae based on cryo-EM reconstruction of the non-contractile sheath/tube complex
Method: EM (helical sym.) / Resolution: 3.7 Å

3567

5myu

EMDB-3567, PDB-5myu:
VipA-N2/VipB contracted sheath of type VI secretion system
Method: EM (helical sym.) / Resolution: 4.0 Å

Source
  • vibrio cholerae (bacteria)
KeywordsTRANSPORT PROTEIN / Type IV secretion system / protein export / PROTEIN TRANSPORT / Bacterial type VI secretion system / STRUCTURAL PROTEIN / T6SS / extended conformation / vibrio cholerae

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