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Structure paper

TitleComplex pectin metabolism by gut bacteria reveals novel catalytic functions.
Journal, issue, pagesNature, Vol. 544, Page 65-70, Year 2017
Publish dateDec 20, 2016 (structure data deposition date)
AuthorsNdeh, D. / Rogowski, A. / Cartmell, A. / Luis, A.S. / Basle, A. / Gray, J. / Venditto, I. / Briggs, J. / Zhang, X. / Labourel, A. ...Ndeh, D. / Rogowski, A. / Cartmell, A. / Luis, A.S. / Basle, A. / Gray, J. / Venditto, I. / Briggs, J. / Zhang, X. / Labourel, A. / Terrapon, N. / Buffetto, F. / Nepogodiev, S. / Xiao, Y. / Field, R.A. / Zhu, Y. / O'Neill, M.A. / Urbanowicz, B.R. / York, W.S. / Davies, G.J. / Abbott, D.W. / Ralet, M.C. / Martens, E.C. / Henrissat, B. / Gilbert, H.J.
External linksNature / PubMed:28329766
MethodsX-ray diffraction
Resolution1.37 - 3 Å
Structure data

PDB-5mqm:
Glycoside hydrolase BT_0986
Method: X-RAY DIFFRACTION / Resolution: 2.11 Å

PDB-5mqn:
Glycoside hydrolase BT_0986
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-5mqo:
Glycoside hydrolase BT_1003
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-5mqr:
Sialidase BT_1020
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-5mqs:
Sialidase BT_1020
Method: X-RAY DIFFRACTION / Resolution: 3 Å

PDB-5msx:
Glycoside hydrolase BT_3662
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-5msy:
Glycoside hydrolase BT_1012
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-5mt2:
Glycoside hydrolase BT_0996
Method: X-RAY DIFFRACTION / Resolution: 1.41 Å

PDB-5mui:
Glycoside hydrolase BT_0996
Method: X-RAY DIFFRACTION / Resolution: 1.77 Å

PDB-5muj:
BT0996 RGII Chain B Complex
Method: X-RAY DIFFRACTION / Resolution: 1.37 Å

PDB-5mwk:
Glycoside hydrolase BT_0986
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-RPQ:
D-rhamnopyranose tetrazole

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-HOH:
WATER

ChemComp-EPE:
4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID / pH buffer*YM

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-NA:
Unknown entry

ChemComp-AHR:
alpha-L-arabinofuranose

ChemComp-144:
TRIS-HYDROXYMETHYL-METHYL-AMMONIUM

ChemComp-PO4:
PHOSPHATE ION

ChemComp-NH3:
AMMONIA

ChemComp-GOL:
GLYCEROL

ChemComp-ACT:
ACETATE ION

ChemComp-IOD:
IODIDE ION

ChemComp-BR:
BROMIDE ION

Source
  • bacteroides thetaiotaomicron (bacteria)
  • bacteroides thetaiotaomicron vpi-5482 (bacteria)
  • bacteroides thetaiotaomicron (strain atcc 29148 / dsm 2079 / nctc 10582 / e50 / vpi-5482) (bacteria)
KeywordsHYDROLASE / glycoside hydrolase / rhamnosidase / plant pectin / CAZy family 106 / aceric acidase / CAZy family GH127 / sialidase / arabinofuranosidase / GH43 / RGII / Apiosidase

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