Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	The Structure of the Plakin Domain of Plectin Reveals an Extended Rod-like Shape.
Journal, issue, pages	J Biol Chem, Vol. 291, Issue 36, Page 18643-18662, Year 2016
Publish date	Sep 2, 2016
Authors	Esther Ortega / José A Manso / Rubén M Buey / Ana M Carballido / Arturo Carabias / Arnoud Sonnenberg / José M de Pereda /
PubMed Abstract	Plakins are large multi-domain proteins that interconnect cytoskeletal structures. Plectin is a prototypical plakin that tethers intermediate filaments to membrane-associated complexes. Most plakins ...Plakins are large multi-domain proteins that interconnect cytoskeletal structures. Plectin is a prototypical plakin that tethers intermediate filaments to membrane-associated complexes. Most plakins contain a plakin domain formed by up to nine spectrin repeats (SR1-SR9) and an SH3 domain. The plakin domains of plectin and other plakins harbor binding sites for junctional proteins. We have combined x-ray crystallography with small angle x-ray scattering (SAXS) to elucidate the structure of the plakin domain of plectin, extending our previous analysis of the SR1 to SR5 region. Two crystal structures of the SR5-SR6 region allowed us to characterize its uniquely wide inter-repeat conformational variability. We also report the crystal structures of the SR7-SR8 region, refined to 1.8 Å, and the SR7-SR9 at lower resolution. The SR7-SR9 region, which is conserved in all other plakin domains, forms a rigid segment stabilized by uniquely extensive inter-repeat contacts mediated by unusually long helices in SR8 and SR9. Using SAXS we show that in solution the SR3-SR6 and SR7-SR9 regions are rod-like segments and that SR3-SR9 of plectin has an extended shape with a small central kink. Other plakins, such as bullous pemphigoid antigen 1 and microtubule and actin cross-linking factor 1, are likely to have similar extended plakin domains. In contrast, desmoplakin has a two-segment structure with a central flexible hinge. The continuous versus segmented structures of the plakin domains of plectin and desmoplakin give insight into how different plakins might respond to tension and transmit mechanical signals.
External links	J Biol Chem / PubMed:27413182 / PubMed Central
Methods	SAS (X-ray synchrotron) / X-ray diffraction
Resolution	1.8 - 3 Å
Structure data	SASDBA4: Plakin domain fragment of Human plectin (central region spectrin repeats: SR3-SR4-SR5-SR6 and SH3) Method: SAXS/SANS SASDBB4: Plakin domain fragment of Human plectin (C-terminal region spectrin repeats: SR7-SR8-SR9) Method: SAXS/SANS SASDBC4: Plakin domain of Human plectin (spectrin repeats: SR3-SR9) Method: SAXS/SANS SASDBD4: Plakin domain fragment of Human desmoplakin (C-terminal region spectrin repeats: SR7-SR8-SR9) Method: SAXS/SANS SASDBE4: Plakin domain of Human desmoplakin (Plakin domain of Human Desmoplakin, Desmoplakin) Method: SAXS/SANS PDB-5j1f: Structure of the spectrin repeats 5 and 6 of the plakin domain of plectin Method: X-RAY DIFFRACTION / Resolution: 3 Å PDB-5j1g: Structure of the spectrin repeats 7 and 8 of the plakin domain of plectin Method: X-RAY DIFFRACTION / Resolution: 1.8 Å PDB-5j1h: Structure of the spectrin repeats 5 and 6 of the plakin domain of plectin Method: X-RAY DIFFRACTION / Resolution: 2.8 Å PDB-5j1i: Structure of the spectrin repeats 7, 8, and 9 of the plakin domain of plectin Method: X-RAY DIFFRACTION / Resolution: 2.801 Å
Chemicals	ChemComp-1PE: PENTAETHYLENE GLYCOL / precipitantYM ChemComp-EDO: 1,2-ETHANEDIOL ChemComp-HOH*: WATER
Source	homo sapiens (human)
Keywords	STRUCTURAL PROTEIN / CYTOSKELETON / PLAKIN / INTERMEDIATE FILAMENT