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Structure paper

TitleCold-induced aldimine bond cleavage by Tris in Bacillus subtilis alanine racemase.
Journal, issue, pagesOrg. Biomol. Chem., Vol. 17, Page 4350-4358, Year 2019
Publish dateMar 14, 2016 (structure data deposition date)
AuthorsBernardo-Garcia, N. / Sanchez-Murcia, P.A. / Espaillat, A. / Martinez-Caballero, S. / Cava, F. / Hermoso, J.A. / Gago, F.
External linksOrg. Biomol. Chem. / PubMed:30977502
MethodsX-ray diffraction
Resolution1.92 - 3 Å
Structure data

PDB-5irp:
Crystal structure of the alanine racemase Bsu17640 from Bacillus subtilis
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-6q70:
Crystal structure of the alanine racemase Bsu17640 from Bacillus subtilis in the presence of HEPES
Method: X-RAY DIFFRACTION / Resolution: 2.05 Å

PDB-6q71:
Crystal structure of the alanine racemase Bsu17640 from Bacillus subtilis in the presence of Bis-Tris propane
Method: X-RAY DIFFRACTION / Resolution: 1.92 Å

PDB-6q72:
Crystal structure of the alanine racemase from Bacillus subtilis in the presence of only PEG 4000 and Magnesium chloride in the crystallization condition
Method: X-RAY DIFFRACTION / Resolution: 3 Å

Chemicals

ChemComp-CL:
Unknown entry

ChemComp-UAH:
(5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-GOL:
GLYCEROL

ChemComp-MG:
Unknown entry

ChemComp-CO2:
CARBON DIOXIDE

ChemComp-HOH:
WATER

ChemComp-PLP:
PYRIDOXAL-5'-PHOSPHATE

Source
  • bacillus subtilis (strain 168) (bacteria)
  • bacillus subtilis (bacteria)
KeywordsISOMERASE / racemase / PLP / alanine racemase / molecular modeling

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