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Structure paper

TitleStructural basis for promiscuous PAM recognition in type I-E Cascade from E. coli.
Journal, issue, pagesNature, Vol. 530, Page 499-503, Year 2016
Publish dateDec 28, 2015 (structure data deposition date)
AuthorsHayes, R.P. / Xiao, Y. / Ding, F. / van Erp, P.B. / Rajashankar, K. / Bailey, S. / Wiedenheft, B. / Ke, A.
External linksNature / PubMed:26863189
MethodsX-ray diffraction
Resolution2.45 - 3.21 Å
Structure data

PDB-5h9e:
Crystal structure of E. coli Cascade bound to a PAM-containing dsDNA target (32-nt spacer) at 3.20 angstrom resolution.
Method: X-RAY DIFFRACTION / Resolution: 3.21 Å

PDB-5h9f:
Crystal structure of E. coli Cascade bound to a PAM-containing dsDNA target at 2.45 angstrom resolution.
Method: X-RAY DIFFRACTION / Resolution: 2.45 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-HOH:
WATER

Source
  • escherichia coli (strain k12) (bacteria)
  • escherichia coli (E. coli)
KeywordsImmune System/RNA / CRISPR Cascade / Immune System-RNA Complex

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