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Structure paper

TitleA structural, functional, and computational analysis suggests pore flexibility as the base for the poor selectivity of CNG channels.
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 112, Page E3619-E3628, Year 2015
Publish dateAug 20, 2014 (structure data deposition date)
AuthorsNapolitano, L.M. / Bisha, I. / De March, M. / Marchesi, A. / Arcangeletti, M. / Demitri, N. / Mazzolini, M. / Rodriguez, A. / Magistrato, A. / Onesti, S. ...Napolitano, L.M. / Bisha, I. / De March, M. / Marchesi, A. / Arcangeletti, M. / Demitri, N. / Mazzolini, M. / Rodriguez, A. / Magistrato, A. / Onesti, S. / Laio, A. / Torre, V.
External linksProc. Natl. Acad. Sci. USA / PubMed:26100907
MethodsX-ray diffraction
Resolution2.3 - 2.85 Å
Structure data

PDB-4r50:
Crystal Structure of CNG mimicking NaK-ETPP mutant cocrystallized with Li+
Method: X-RAY DIFFRACTION / Resolution: 2.85 Å

PDB-4r6z:
Crystal Structure of CNG mimicking NaK mutant, NaK-ETPP, Cs+ complex
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-4r7c:
Crystal Structure of CNG mimicking NaK-ETPP mutant cocrystallized with DiMethylammonium
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-4r8c:
Crystal Structure of CNG mimicking NaK-ETPP mutant in complex with Rb+
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-4rai:
Crystal Structure of CNG mimicking NaK-ETPP mutant in complex with Na+
Method: X-RAY DIFFRACTION / Resolution: 2.31 Å

PDB-4ro2:
Crystal Structure of CNG mimicking NaK-ETPP mutant cocrystallized with Methylammonium
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

Chemicals

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

ChemComp-GLY:
GLYCINE

ChemComp-HOH:
WATER

ChemComp-CS:
Unknown entry

ChemComp-DMN:
DIMETHYLAMINE

ChemComp-RB:
RUBIDIUM ION

ChemComp-NA:
Unknown entry

ChemComp-3P8:
methylammonium ion

Source
  • bacillus cereus atcc 14579 (bacteria)
KeywordsTRANSPORT PROTEIN / Alpha helical membrane protein / chimera channel / ALPHA-HELICAL membrane protein / NaK-chimera channel in complex with DiMA+ / ion channel

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