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- PDB-4r7c: Crystal Structure of CNG mimicking NaK-ETPP mutant cocrystallized... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4r7c | ||||||
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Title | Crystal Structure of CNG mimicking NaK-ETPP mutant cocrystallized with DiMethylammonium | ||||||
![]() | Potassium channel protein | ||||||
![]() | TRANSPORT PROTEIN / ALPHA-HELICAL membrane protein / NaK-chimera channel in complex with DiMA+ | ||||||
Function / homology | ![]() stabilization of membrane potential / potassium ion leak channel activity / outward rectifier potassium channel activity / identical protein binding / membrane / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | De March, M. / Napolitano, L.M.R. / Onesti, S. | ||||||
![]() | ![]() Title: A structural, functional, and computational analysis suggests pore flexibility as the base for the poor selectivity of CNG channels. Authors: Napolitano, L.M. / Bisha, I. / De March, M. / Marchesi, A. / Arcangeletti, M. / Demitri, N. / Mazzolini, M. / Rodriguez, A. / Magistrato, A. / Onesti, S. / Laio, A. / Torre, V. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 89.7 KB | Display | ![]() |
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PDB format | ![]() | 68.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 511.3 KB | Display | ![]() |
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Full document | ![]() | 527.4 KB | Display | |
Data in XML | ![]() | 20.8 KB | Display | |
Data in CIF | ![]() | 26.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4r50C ![]() 4r6zC ![]() 4r8cC ![]() 4raiC ![]() 4ro2C ![]() 3k0dS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Details | The biological tetramer is assembled by a crystallographic 2-fold axis which relates two dimers together. The pore of the tetrameric channel is coincident with the 2-fold crystallographic axis. |
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Components
#1: Protein | Mass: 10626.555 Da / Num. of mol.: 4 / Fragment: residues 20-110 / Mutation: D66E, G67T, N68P, F69P Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-GLY / #3: Chemical | ChemComp-MPD / ( #4: Chemical | ChemComp-DMN / #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.42 Å3/Da / Density % sol: 49.16 % |
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Crystal grow | pH: 6.5 Details: CNG-ETPP(DiMA+) cocrystals grown in 40-44% MPD, 100mM MES pH6.5 and 20-25 mM Glycine, VAPOR DIFFUSION, HANGING DROP |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 2M / Detector: PIXEL / Date: May 6, 2014 |
Radiation | Monochromator: SI 111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→47.84 Å / Num. obs: 18763 / % possible obs: 99.6 % / Observed criterion σ(I): 2.1 |
Reflection shell | Resolution: 2.3→47.84 Å / Rmerge(I) obs: 0.071 / Mean I/σ(I) obs: 4.3 / % possible all: 99.6 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3K0D Resolution: 2.3→47.84 Å / σ(F): 2.1 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.3→47.84 Å
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Refine LS restraints |
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