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Structure paper

TitleStructures of Human Steroidogenic Cytochrome P450 17A1 with Substrates.
Journal, issue, pagesJ. Biol. Chem., Vol. 289, Page 32952-32964, Year 2014
Publish dateNov 13, 2013 (structure data deposition date)
AuthorsPetrunak, E.M. / DeVore, N.M. / Porubsky, P.R. / Scott, E.E.
External linksJ. Biol. Chem. / PubMed:25301938
MethodsX-ray diffraction
Resolution2.5 - 3.003 Å
Structure data

PDB-4nkv:
Human steroidogenic cytochrome P450 17A1 mutant A105L with inhibitor abiraterone
Method: X-RAY DIFFRACTION / Resolution: 2.646 Å

PDB-4nkw:
Human steroidogenic cytochrome P450 17A1 mutant A105L with substrate pregnenolone
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-4nkx:
Human steroidogenic cytochrome P450 17A1 mutant A105L with substrate progesterone
Method: X-RAY DIFFRACTION / Resolution: 2.794 Å

PDB-4nky:
Human steroidogenic cytochrome P450 17A1 mutant A105L with substrate 17alpha-hydroxyprogesterone
Method: X-RAY DIFFRACTION / Resolution: 2.55 Å

PDB-4nkz:
Human steroidogenic cytochrome P450 17A1 mutant A105L with substrate 17alpha-hydroxypregnenolone
Method: X-RAY DIFFRACTION / Resolution: 3.003 Å

Chemicals

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-AER:
Abiraterone / medication*YM

ChemComp-HOH:
WATER

ChemComp-PLO:
(3BETA)-3-HYDROXYPREGN-5-EN-20-ONE / medication, hormone*YM

ChemComp-STR:
PROGESTERONE / hormone*YM

ChemComp-3QZ:
(9beta)-17-hydroxypregn-4-ene-3,20-dione / hormone*YM

ChemComp-LZZ:
(3alpha,8alpha)-3,17-dihydroxypregn-5-en-20-one

Source
  • homo sapiens (human)
KeywordsOXIDOREDUCTASE / LYASE/INHIBITOR / heme protein / monooxygenase / steroid 17alpha-hydroxylase / steroid C17 / 20 lyase / NADPH-cytochrome P450 reductase / cytochrome b5 / endoplasmic reticulum membrane / LYASE-INHIBITOR complex / LYASE

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