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TitleStructural analysis of monomeric retroviral reverse transcriptase in complex with an RNA/DNA hybrid.
Journal, issue, pagesNucleic Acids Res, Vol. 41, Issue 6, Page 3874-3887, Year 2013
Publish dateApr 1, 2013
AuthorsElzbieta Nowak / Wojciech Potrzebowski / Petr V Konarev / Jason W Rausch / Marion K Bona / Dmitri I Svergun / Janusz M Bujnicki / Stuart F J Le Grice / Marcin Nowotny /
PubMed AbstractA key step in proliferation of retroviruses is the conversion of their RNA genome to double-stranded DNA, a process catalysed by multifunctional reverse transcriptases (RTs). Dimeric and monomeric ...A key step in proliferation of retroviruses is the conversion of their RNA genome to double-stranded DNA, a process catalysed by multifunctional reverse transcriptases (RTs). Dimeric and monomeric RTs have been described, the latter exemplified by the enzyme of Moloney murine leukaemia virus. However, structural information is lacking that describes the substrate binding mechanism for a monomeric RT. We report here the first crystal structure of a complex between an RNA/DNA hybrid substrate and polymerase-connection fragment of the single-subunit RT from xenotropic murine leukaemia virus-related virus, a close relative of Moloney murine leukaemia virus. A comparison with p66/p51 human immunodeficiency virus-1 RT shows that substrate binding around the polymerase active site is conserved but differs in the thumb and connection subdomains. Small-angle X-ray scattering was used to model full-length xenotropic murine leukaemia virus-related virus RT, demonstrating that its mobile RNase H domain becomes ordered in the presence of a substrate-a key difference between monomeric and dimeric RTs.
External linksNucleic Acids Res / PubMed:23382176 / PubMed Central
MethodsSAS (X-ray synchrotron) / X-ray diffraction
Resolution3.04 Å
Structure data

SASDAV5:
apo XMRV RT (XM)
Method: SAXS/SANS

SASDAW5:
XMRV RT + DNA/RNA hybrid (apo XMRV RT, XM + RNA_DNA hybrid substrate, PPT18)
Method: SAXS/SANS

PDB-4hkq:
XMRV reverse transcriptase in complex with RNA/DNA hybrid
Method: X-RAY DIFFRACTION / Resolution: 3.04 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • Escherichia coli (E. coli)
  • xenotropic mulv-related virus
KeywordsTRANSCRIPTION/RNA/DNA / Protein-DNA-RNA complex / reverse transcription / TRANSCRIPTION-RNA-DNA complex

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