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Structure paper

TitleCrystal Structures of Physcomitrella patens AOC1 and AOC2: Insights into the Enzyme Mechanism and Differences in Substrate Specificity.
Journal, issue, pagesPlant Physiol., Vol. 160, Page 1251-1266, Year 2012
Publish dateSep 19, 2012 (structure data deposition date)
AuthorsNeumann, P. / Brodhun, F. / Sauer, K. / Herrfurth, C. / Hamberg, M. / Brinkmann, J. / Scholz, J. / Dickmanns, A. / Feussner, I. / Ficner, R.
External linksPlant Physiol. / PubMed:22987885
MethodsX-ray diffraction
Resolution1.35 - 2 Å
Structure data

PDB-4h69:
Crystal Structure of the Allene Oxide Cyclase 2 from Physcomitrella patens complexed with substrate analog
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-4h6a:
Crystal Structure of the Allene Oxide Cyclase 2 from Physcomitrella patens
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

PDB-4h6b:
Structural basis for allene oxide cyclization in moss
Method: X-RAY DIFFRACTION / Resolution: 1.35 Å

PDB-4h6c:
Crystal Structure of the Allene Oxide Cyclase 1 from Physcomitrella patens
Method: X-RAY DIFFRACTION / Resolution: 1.35 Å

Chemicals

ChemComp-PO4:
PHOSPHATE ION

ChemComp-10Y:
(9Z)-11-{(2R,3S)-3-[(2Z)-pent-2-en-1-yl]oxiran-2-yl}undec-9-enoic acid

ChemComp-IPA:
ISOPROPYL ALCOHOL / alkaloid*YM

ChemComp-HOH:
WATER

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

ChemComp-SO4:
SULFATE ION

ChemComp-MRD:
(4R)-2-METHYLPENTANE-2,4-DIOL / precipitant*YM

ChemComp-10X:
(9Z)-11-{(2S,3S)-3-[(2Z)-pent-2-en-1-yl]oxiran-2-yl}undec-9-enoic acid

ChemComp-HEZ:
HEXANE-1,6-DIOL

Source
  • physcomitrella patens (plant)
  • physcomitrella patens subsp. patens (plant)
KeywordsISOMERASE / B-barrel / Oxylipins / fatty acid / metabolites / allene-oxide cyclase activity

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