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Structure paper

TitleThe structure of the COPII transport-vesicle coat assembled on membranes.
Journal, issue, pagesElife, Vol. 2, Page e00951, Year 2013
Publish dateSep 17, 2013
AuthorsGiulia Zanetti / Simone Prinz / Sebastian Daum / Annette Meister / Randy Schekman / Kirsten Bacia / John A G Briggs /
PubMed AbstractCoat protein complex II (COPII) mediates formation of the membrane vesicles that export newly synthesised proteins from the endoplasmic reticulum. The inner COPII proteins bind to cargo and membrane, ...Coat protein complex II (COPII) mediates formation of the membrane vesicles that export newly synthesised proteins from the endoplasmic reticulum. The inner COPII proteins bind to cargo and membrane, linking them to the outer COPII components that form a cage around the vesicle. Regulated flexibility in coat architecture is essential for transport of a variety of differently sized cargoes, but structural data on the assembled coat has not been available. We have used cryo-electron tomography and subtomogram averaging to determine the structure of the complete, membrane-assembled COPII coat. We describe a novel arrangement of the outer coat and find that the inner coat can assemble into regular lattices. The data reveal how coat subunits interact with one another and with the membrane, suggesting how coordinated assembly of inner and outer coats can mediate and regulate packaging of vesicles ranging from small spheres to large tubular carriers. DOI:http://dx.doi.org/10.7554/eLife.00951.001.
External linksElife / PubMed:24062940 / PubMed Central
MethodsEM (subtomogram averaging) / EM (tomography)
Resolution23.0 - 40.0 Å
Structure data

2428

4bzi

EMDB-2428, PDB-4bzi:
The structure of the COPII coat assembled on membranes
Method: EM (subtomogram averaging) / Resolution: 23.0 Å

EMDB-2429:
The structure of the COPII coat assembled on membranes
Method: EM (subtomogram averaging) / Resolution: 40.0 Å

2430

4bzj

EMDB-2430, PDB-4bzj:
The structure of the COPII coat assembled on membranes
Method: EM (subtomogram averaging) / Resolution: 40.0 Å

2431

4bzk

EMDB-2431, PDB-4bzk:
The structure of the COPII coat assembled on membranes
Method: EM (subtomogram averaging) / Resolution: 40.0 Å

EMDB-2432:
The structure of the COPII coat assembled on membranes
Method: EM (tomography)

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-GNP:
PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / GppNHp, GMPPNP, energy-carrying molecule analogue*YM

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

Source
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsTRANSPORT PROTEIN / SECRETION / TRAFFICKING / SEC23 / SEC24 / SAR1 / PROTEIN TRANSPORT

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