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TitleStructure and superorganization of acetylcholine receptor-rapsyn complexes.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 110, Issue 26, Page 10622-10627, Year 2013
Publish dateJun 25, 2013
AuthorsBenoît Zuber / Nigel Unwin /
PubMed AbstractThe scaffolding protein at the neuromuscular junction, rapsyn, enables clustering of nicotinic acetylcholine receptors in high concentration and is critical for muscle function. Patients with ...The scaffolding protein at the neuromuscular junction, rapsyn, enables clustering of nicotinic acetylcholine receptors in high concentration and is critical for muscle function. Patients with insufficient receptor clustering suffer from muscle weakness. However, the detailed organization of the receptor-rapsyn network is poorly understood: it is unclear whether rapsyn first forms a wide meshwork to which receptors can subsequently dock or whether it only forms short bridges linking receptors together to make a large cluster. Furthermore, the number of rapsyn-binding sites per receptor (a heteropentamer) has been controversial. Here, we show by cryoelectron tomography and subtomogram averaging of Torpedo postsynaptic membrane that receptors are connected by up to three rapsyn bridges, the minimum number required to form a 2D network. Half of the receptors belong to rapsyn-connected groups comprising between two and fourteen receptors. Our results provide a structural basis for explaining the stability and low diffusion of receptors within clusters.
External linksProc Natl Acad Sci U S A / PubMed:23754381 / PubMed Central
MethodsEM (subtomogram averaging) / EM (tomography)
Resolution40 - 50 Å
Structure data

2376

4bog

EMDB-2376, PDB-4bog:
The structure and super-organization of acetylcholine receptor-rapsyn complexes
Method: EM (subtomogram averaging)

2377

4boi

EMDB-2377: The structure and super-organization of acetylcholine receptor-rapsyn complexes; class A
PDB-4boi: The structure and super-organization of acetylcholine receptor-rapsyn complexes class A
Method: EM (subtomogram averaging) / Resolution: 41.0 Å

2378

4bon

EMDB-2378: The structure and super-organization of acetylcholine receptor-rapsyn complexes; class B
PDB-4bon: The structure and super-organization of acetylcholine receptor-rapsyn complexes class B
Method: EM (subtomogram averaging) / Resolution: 41.0 Å

2381

4boo

EMDB-2381: The structure and super-organization of acetylcholine receptor-rapsyn complexes; class C
PDB-4boo: The structure and super-organization of acetylcholine receptor-rapsyn complexes class C
Method: EM (subtomogram averaging) / Resolution: 42.0 Å

2382

4bor

EMDB-2382: The structure and super-organization of acetylcholine receptor-rapsyn complexes; class D
PDB-4bor: The structure and super-organization of acetylcholine receptor-rapsyn complexes class D
Method: EM (subtomogram averaging) / Resolution: 42.0 Å

2383

4bot

EMDB-2383: The structure and super-organization of acetylcholine receptor-rapsyn complexes; class E
PDB-4bot: The structure and super-organization of acetylcholine receptor- rapsyn complexes class E
Method: EM (subtomogram averaging) / Resolution: 42.0 Å

Source
  • torpedo marmorata (marbled electric ray)
KeywordsTRANSPORT PROTEIN / CLUSTERING / SYNAPSE / NEUROMUSCULAR JUNCTION / NICOTINIC / RAPSYN / 43K / ELECTRIC ORGAN / NEUROTRANSMITTER RECEPTOR / RECEPTOR

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