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Structure paper

TitleThe Structure of E. Coli Exoix - Implications for DNA Binding and Catalysis in Flap Endonucleases
Journal, issue, pagesNucleic Acids Res., Vol. 41, Page 8357-, Year 2013
Publish dateNov 26, 2012 (structure data deposition date)
AuthorsAnstey-Gilbert, C.S. / Hemsworth, G.R. / Flemming, C.S. / Hodskinson, M.R.G. / Zhang, J. / Sedelnikova, S.E. / Stillman, T.J. / Sayers, J.R. / Artymiuk, P.J.
External linksNucleic Acids Res. / PubMed:23821668
MethodsX-ray diffraction
Resolution1.47 - 2.45 Å
Structure data

PDB-3zd8:
Potassium bound structure of E. coli ExoIX in P1
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-3zd9:
Potassium bound structure of E. coli ExoIX in P21
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-3zda:
Structure of E. coli ExoIX in complex with a fragment of the Flap1 DNA oligonucleotide, potassium and magnesium
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-3zdb:
Structure of E. coli ExoIX in complex with the palindromic 5ov4 DNA oligonucleotide, di-magnesium and potassium
Method: X-RAY DIFFRACTION / Resolution: 1.47 Å

PDB-3zdc:
Structure of E. coli ExoIX in complex with the palindromic 5ov4 DNA oligonucleotide, potassium and calcium
Method: X-RAY DIFFRACTION / Resolution: 1.53 Å

PDB-3zdd:
Structure of E. coli ExoIX in complex with the palindromic 5ov6 oligonucleotide and potassium
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-3zde:
Potassium free structure of E. coli ExoIX
Method: X-RAY DIFFRACTION / Resolution: 2.45 Å

Chemicals

ChemComp-K:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-MG:
Unknown entry

ChemComp-PIV:
PIVALIC ACID

ChemComp-PO4:
PHOSPHATE ION

ChemComp-ACT:
ACETATE ION

ChemComp-CA:
Unknown entry

ChemComp-IPA:
ISOPROPYL ALCOHOL / alkaloid*YM

ChemComp-EDO:
1,2-ETHANEDIOL

Source
  • escherichia coli (E. coli)
  • synthetic construct (others)
KeywordsHYDROLASE / FLAP ENDONUCLEASE / DNA BINDING / HYDROLASE/DNA / HYDROLASE-DNA COMPLEX / EXOIX / ENDONUCLEASE

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