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TitleTemperature-dependent ligand relocation reveals plasticity of TRPM4 inhibition.
Journal, issue, pagesbioRxiv, Year 2026
Publish dateMay 14, 2026
AuthorsDominic Schneiter / Jean-Sébastien Rougier / Hugues Abriel / Henning Stahlberg / Babatunde Ekundayo
PubMed AbstractTransient receptor potential melastatin 4 (TRPM4) is a Ca²⁺-activated cation channel whose pharmacology is shaped by its molecular environment. It remains poorly understood how temperature and ...Transient receptor potential melastatin 4 (TRPM4) is a Ca²⁺-activated cation channel whose pharmacology is shaped by its molecular environment. It remains poorly understood how temperature and membrane context influence inhibitor recognition. Here we combine cryo-electron microscopy of membrane-derived vesicles and detergent-solubilized TRPM4 to investigate lipid-associated architecture and binding of the potent anthranilic anilide inhibitor PBA. We find that membrane vesicles preserve a native-like paralipid environment and reveal lipid binding patterns highly similar to those observed in GDN, supporting detergent-solubilized TRPM4 as a structurally relevant system for ligand analysis. Strikingly, PBA occupies distinct binding pockets at 8□°C and 37□°C. At low temperature, PBA binds in a previously described inhibitor pocket formed by S3, S4, the S4-S5 linker and the TRP helix, whereas at physiological temperature it relocates to a distinct site within the S1-S4 domain proximal to the Ca²⁺ regulatory region. These findings reveal temperature-dependent plasticity in TRPM4 ligand recognition.
External linksbioRxiv / PubMed:42182356 / PubMed Central
MethodsEM (single particle)
Resolution3.0 - 3.4 Å
Structure data

57864

30kw

EMDB-57864, PDB-30kw:
Human Trpm4 at 8 degrees Celsius
Method: EM (single particle) / Resolution: 3.1 Å

57865

30kz

EMDB-57865, PDB-30kz:
Human trpm4 in complex with PBA at 8 degrees Celsius
Method: EM (single particle) / Resolution: 3.4 Å

57866

30la

EMDB-57866, PDB-30la:
Human Trpm4 with calcium at 37 degrees celsius
Method: EM (single particle) / Resolution: 3.0 Å

57879

30ld

EMDB-57879, PDB-30ld:
Human Trpm4 with calcium and PBA at 37 degrees celsius
Method: EM (single particle) / Resolution: 3.0 Å

Chemicals

ChemComp-CLR:
CHOLESTEROL

PDB-1j74:
Crystal Structure of Mms2

ChemComp-CA:
Unknown entry

Source
  • homo sapiens (human)
KeywordsMEMBRANE PROTEIN / TRP channel / ion channel

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