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Structure paper

TitleStructural Snapshots of beta-1,4-Galactosyltransferase-I Along the Kinetic Pathway.
Journal, issue, pagesJ. Mol. Biol., Vol. 357, Page 1619-1633, Year 2006
Publish dateFeb 7, 2006 (structure data deposition date)
AuthorsRamakrishnan, B. / Ramasamy, V. / Qasba, P.K.
External linksJ. Mol. Biol. / PubMed:16497331
MethodsX-ray diffraction
Resolution1.7 - 2 Å
Structure data

PDB-2fy7:
Crystal structure of the catalytic domain of the human beta1,4-galactosyltransferase mutant M339H in apo form
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-2fya:
Crystal structure of the catalytic domain of the human beta1, 4-galactosyltransferase mutant M339H complex with manganese
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-2fyb:
Crystal structure of the catalytic domain of the human beta1,4-galactosyltransferase mutant M339H in complex with Mn and UDP-galactose in open conformation
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-2fyc:
Crystal structure of the catalytic domain of bovine beta1,4-galactosyltransferase-I in complex with alpha-lactalbumin, Ca and UDP-galactose
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-2fyd:
catalytic domain of bovine beta 1, 4-galactosyltransferase in complex with alpha-lactalbumin, glucose, Mn, and UDP-N-acetylgalactosamine
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-HOH:
WATER

ChemComp-MN:
Unknown entry

ChemComp-UDP:
URIDINE-5'-DIPHOSPHATE / UDP*YM

ChemComp-CA:
Unknown entry

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM

ChemComp-GDU:
GALACTOSE-URIDINE-5'-DIPHOSPHATE

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-BGC:
beta-D-glucopyranose

ChemComp-NGA:
2-acetamido-2-deoxy-beta-D-galactopyranose

Source
  • homo sapiens (human)
  • mus musculus (house mouse)
  • bos taurus (cattle)
KeywordsTRANSFERASE / M339H mutant / apo enzyme / Mn binding / complex with UDP-galactose and Mn / open conformation / lactose synthase / M344H mutation / Ca binding / lactose sythase / catalytic intermediate

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