Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Atypical signaling, ligand recognition and selective agonist discovery of complement receptor C5aR2.
Journal, issue, pages	Cell Res., Year 2026
Publish date	Jun 2, 2026
Authors	Jiao Qin / Chenxi Cai / Mayu Shan / Suqing Zhou / Qingya Shen / Tianyi Zhu / Mingming Zhao / Yang Mei / Fanghan Ji / Dan-Dan Shen / Shao-Kun Zang / Huibing Zhang / Haomang Xu / Ming Yang / Wei-Wei Wang / Rong Xiao / Bing Yang / Chunyou Mao / Zhenhua Shao / Haijing Wu / Qianjin Lu / Yan Zhang /
PubMed Abstract	C5a, the most potent anaphylatoxin in the complement system, exerts its effects through the canonical G protein-coupled receptor C5aR1 and the arrestin-coupled receptor C5aR2. Despite the critical ...C5a, the most potent anaphylatoxin in the complement system, exerts its effects through the canonical G protein-coupled receptor C5aR1 and the arrestin-coupled receptor C5aR2. Despite the critical role of C5aR2 in immunomodulation, the molecular mechanisms underlying its biased signaling, ligand recognition, and associated pathophysiology remain poorly understood. Here, we report cryo-electron microscopy structures of β-arrestin 1-bound C5aR2 and C5aR1 stimulated by C5a or its metabolite C5a. By combining structural analysis with functional assays, we identified the key structural determinants that prevent G protein coupling and confer intrinsic bias toward β-arrestins. Comparative analysis elucidated the distinct ligand recognition mechanism of C5aR2 and explained the retained affinity of C5a for C5aR2. These findings guided the rational design of ZQ105, a highly selective C5aR2 agonist. Leveraging ZQ105 as a chemical probe, functional studies revealed that selective C5aR2 activation induces distinct pro-inflammatory responses and receptor internalization in neutrophils. This study provides novel structural insights into transducer engagement and ligand recognition by C5aR2, yielding a valuable pharmacological tool for exploring C5aR2-related pathophysiological processes.
External links	Cell Res. / PubMed:42399467
Methods	EM (single particle)
Resolution	2.7 - 3.3 Å
Structure data	68177 EMDB Unreleased entry 22cr EMDB-68177, PDB-22cr: C5a-desArg bound C5aR2 in complex with beta-arrestin1 Method: EM (single particle) / Resolution: 2.9 Å 68262 EMDB Unreleased entry 22gk EMDB-68262, PDB-22gk: C5a bound C5aR2 in complex with beta-arrestin1 Method: EM (single particle) / Resolution: 2.84 Å 68263 EMDB Unreleased entry 22gl EMDB-68263, PDB-22gl: C5a bound C5aR1 in complex with beta-arrestin1 Method: EM (single particle) / Resolution: 3.3 Å 68264 EMDB Unreleased entry 22gm EMDB-68264, PDB-22gm: C5a bound C5aR1 in complex with beta-arrestin1 Method: EM (single particle) / Resolution: 2.7 Å EMDB Unreleased entry EMDB-68516: C5a bound C5aR2 in complex with beta-arrestin1 Method: EM (single particle) / Resolution: 2.84 Å EMDB Unreleased entry EMDB-68517: C5a bound C5aR2 in complex with beta-arrestin1(focused on Receptor) Method: EM (single particle) / Resolution: 3.0 Å EMDB Unreleased entry EMDB-68520: C5a bound C5aR1(V2R C tail) in complex with beta-arrestin1 (Focus on Receptor) Method: EM (single particle) / Resolution: 2.9 Å EMDB Unreleased entry EMDB-68524: C5a bound C5aR1(V2R C tail) in complex with beta-arrestin1 Method: EM (single particle) / Resolution: 2.7 Å EMDB Unreleased entry EMDB-68525: C5a bound C5aR1(V2R C tail) in complex with beta-arrestin1 (Focus on Arrestin) Method: EM (single particle) / Resolution: 2.7 Å EMDB Unreleased entry EMDB-68529: C5a-desArg bound C5aR2 in complex with beta-arrestin1(focus on receptor) Method: EM (single particle) / Resolution: 3.1 Å EMDB Unreleased entry EMDB-68531: C5a-desArg bound C5aR2 in complex with beta-arrestin1 Method: EM (single particle) / Resolution: 3.0 Å EMDB Unreleased entry EMDB-68534: C5a-desArg bound C5aR2 in complex with beta-arrestin1 (Focus on Arrestin) Method: EM (single particle) / Resolution: 2.9 Å EMDB Unreleased entry EMDB-77405: C5a bound C5aR2 in complex with beta-arrestin1 (focused on barr1-Fab30 complex) Method: EM (single particle) / Resolution: 2.8 Å
Source	homo sapiens (human) mus musculus (house mouse) bos taurus (domestic cattle)
Keywords	MEMBRANE PROTEIN/IMMUNE SYSTEM / C5aR2 / GPCR / membrane protein / active / arrestin / C5a-desArg / MEMBRANE PROTEIN-IMMUNE SYSTEM complex / MEMBRANE PROTEIN/IMMUNESYSTEM / C5a / MEMBRANE PROTEIN-IMMUNESYSTEM complex