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TitleCryo-EM reveals an active role for aminoacyl-tRNA in the accommodation process.
Journal, issue, pagesEMBO J, Vol. 21, Issue 13, Page 3557-3567, Year 2002
Publish dateJul 1, 2002
AuthorsMikel Valle / Jayati Sengupta / Neil K Swami / Robert A Grassucci / Nils Burkhardt / Knud H Nierhaus / Rajendra K Agrawal / Joachim Frank /
PubMed AbstractDuring the elongation cycle of protein biosynthesis, the specific amino acid coded for by the mRNA is delivered by a complex that is comprised of the cognate aminoacyl-tRNA, elongation factor Tu and ...During the elongation cycle of protein biosynthesis, the specific amino acid coded for by the mRNA is delivered by a complex that is comprised of the cognate aminoacyl-tRNA, elongation factor Tu and GTP. As this ternary complex binds to the ribosome, the anticodon end of the tRNA reaches the decoding center in the 30S subunit. Here we present the cryo- electron microscopy (EM) study of an Escherichia coli 70S ribosome-bound ternary complex stalled with an antibiotic, kirromycin. In the cryo-EM map the anticodon arm of the tRNA presents a new conformation that appears to facilitate the initial codon-anticodon interaction. Furthermore, the elbow region of the tRNA is seen to contact the GTPase-associated center on the 50S subunit of the ribosome, suggesting an active role of the tRNA in the transmission of the signal prompting the GTP hydrolysis upon codon recognition.
External linksEMBO J / PubMed:12093756 / PubMed Central
MethodsEM (single particle)
Resolution16.8 Å
Structure data

1045

1ls2

1lu3

EMDB-1045: Cryo-EM reveals an active role for aminoacyl-tRNA in the accommodation process.
PDB-1ls2: Fitting of EF-Tu and tRNA in the Low Resolution Cryo-EM Map of an EF-Tu Ternary Complex (GDP and Kirromycin) Bound to E. coli 70S Ribosome
PDB-1lu3: Separate Fitting of the Anticodon Loop Region of tRNA (nucleotide 26-42) in the Low Resolution Cryo-EM Map of an EF-Tu Ternary Complex (GDP and Kirromycin) Bound to E. coli 70S Ribosome
Method: EM (single particle) / Resolution: 16.8 Å

Source
  • escherichia coli (E. coli)
  • synthetic construct (others)
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsTRANSLATION/RNA / EF-Tu / ternary complex / cryo-EM / 70S E.coli ribosome / TRANSLATION-RNA COMPLEX / RNA / tRNA / conformational change

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