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Structure paper

TitleCrystal Structures of Substrate and Inhibitor Complexes with AmpC -Lactamase: Possible Implications for Substrate-Assisted Catalysis
Journal, issue, pagesJ. Am. Chem. Soc., Vol. 122, Page 10504-10512, Year 2000
Publish dateJul 18, 2000 (structure data deposition date)
AuthorsPatera, A. / Blaszczak, L.C. / Shoichet, B.K.
External linksJ. Am. Chem. Soc. / Search PubMed
MethodsX-ray diffraction
Resolution2.2 - 2.46 Å
Structure data

PDB-1fcm:
CRYSTAL STRUCTURE OF THE E.COLI AMPC BETA-LACTAMASE MUTANT Q120L/Y150E COVALENTLY ACYLATED WITH THE INHIBITORY BETA-LACTAM, CLOXACILLIN
Method: X-RAY DIFFRACTION / Resolution: 2.46 Å

PDB-1fcn:
Crystal Structure of the E. Coli AMPC Beta-Lactamase Mutant Q120L/Y150E Covalently Acylated with the Substrate Beta-Lactam LORACARBEF
Method: X-RAY DIFFRACTION / Resolution: 2.35 Å

PDB-1fco:
CRYSTAL STRUCTURE OF THE E. COLI AMPC BETA-LACTAMASE COVALENTLY ACYLATED WITH THE INHIBITORY BETA-LACTAM, MOXALACTAM
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

Chemicals

ChemComp-CXU:
CLOXACILLIN (OPEN FORM)

ChemComp-HOH:
WATER

ChemComp-LOR:
LORACABEF (Open form)

ChemComp-MOX:
(2R)-2-[(1R)-1-{[(2S)-2-carboxy-2-(4-hydroxyphenyl)acetyl]amino}-1-methoxy-2-oxoethyl]-5-methylidene-5,6-dihydro-2H-1,3-oxazine-4-carboxylic acid

Source
  • escherichia coli (E. coli)
Keywordshydrolase/antibiotic / beta-lactamase beta-lactam complex / enzyme inhibitor complex / hydrolase-antibiotic complex / HYDROLASE

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