EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural determination of lipid-bound factor VIII.
ジャーナル・号・ページ	J Thromb Haemost, Year 2026
掲載日	2026年5月26日
著者	Bassem M Mohammed /
PubMed 要旨	BACKGROUND: Coagulation factor (F)VIII is the inactive precursor of FVIIIa, an essential component of the intrinsic tenase complex. Despite numerous structures of recombinant FVIII in solution, the ...BACKGROUND: Coagulation factor (F)VIII is the inactive precursor of FVIIIa, an essential component of the intrinsic tenase complex. Despite numerous structures of recombinant FVIII in solution, the structural basis of the procofactor-membrane interface remains poorly defined. Traditional liposome platforms require special grids and often suffer from heterogeneous particle distribution and overcrowding, precluding high-resolution single-particle analysis, while membrane mimetics-nanodiscs-are too planar, posing significant biophysical challenges. OBJECTIVES: This study established a reproducible methodology for structural determination of membrane-bound coagulation factors and resolve the procofactor FVIII-lipid interface. 手法: We used methylated branched lipids to compensate for membrane planarity and used cryoelectron microscopy to solve the structure of FVIII bound to lipids. RESULTS: We resolved the cryoelectron microscopy structure of procofactor FVIII on nanodiscs and liposomes lipid membranes at 3.46 and 3.56 Å, respectively. Structural analysis reveals a definitive tilted docking orientation where both C domains mediate shallow interfacial insertion, with the C2 domain acting as the primary anchor and the C1 domain serving as a secondary tether. This spatial arrangement structurally explains the high clinical severity of C2 interface mutations compared with more moderate C1 surface mutations. CONCLUSION: Methylated branched lipids enable rapid, high-resolution characterization of membrane-associated clotting factors. This structure provides the first definitive template for the FVIII-lipid interface, serving as a benchmark for future studies on tenase complex assembly.
リンク	J Thromb Haemost / PubMed:42202884
手法	EM (単粒子)
解像度	3.46 - 3.67 Å
構造データ	EMDB-76479: Structural determination of lipid-bound Factor VIII 手法: EM (単粒子) / 解像度: 3.46 Å EMDB-76482: Structural determination of lipid-bound Factor VIII 手法: EM (単粒子) / 解像度: 3.47 Å EMDB-76483: Structural determination of lipid-bound Factor VIII 手法: EM (単粒子) / 解像度: 3.55 Å EMDB-76484: Structural determination of lipid-bound Factor VIII 手法: EM (単粒子) / 解像度: 3.46 Å EMDB-76485: Structural determination of lipid-bound Factor VIII 手法: EM (単粒子) / 解像度: 3.66 Å EMDB-76486: Structural determination of lipid-bound Factor VIII 手法: EM (単粒子) / 解像度: 3.46 Å 76487 12jp EMDB-76487, PDB-12jp: Structural determination of lipid-bound Factor VIII 手法: EM (単粒子) / 解像度: 3.46 Å EMDB-76489: Structural determination of lipid-bound Factor VIII 手法: EM (単粒子) / 解像度: 3.56 Å EMDB-76490: Structural determination of lipid-bound Factor VIII 手法: EM (単粒子) / 解像度: 3.63 Å EMDB-76491: Structural determination of lipid-bound Factor VIII 手法: EM (単粒子) / 解像度: 3.55 Å EMDB-76492: Structural determination of lipid-bound Factor VIII 手法: EM (単粒子) / 解像度: 3.48 Å EMDB-76494: Structural determination of lipid-bound Factor VIII 手法: EM (単粒子) / 解像度: 3.67 Å EMDB-76495: Structural determination of lipid-bound Factor VIII 手法: EM (単粒子) / 解像度: 3.56 Å 76497 12jv EMDB-76497, PDB-12jv: Structural determination of lipid-bound Factor VIII 手法: EM (単粒子) / 解像度: 3.56 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose ChemComp-CA: Unknown entry ChemComp-CU: COPPER (II) ION
由来	homo sapiens (ヒト)
キーワード	BLOOD CLOTTING / Coagulation / Intrinsic Pathway / Hemophilia / Factor VIII