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TitleStructural determinants of endopilus assembly, stability, and functional specificity in bacterial type II secretion.
Journal, issue, pagesStructure, Vol. 34, Issue 10, Page 1324-11335.e5, Year 2026
Publish dateAug 20, 2026
AuthorsMaylis Lejeune / Stefaniia Ivashchenko / Régine Dazzoni / Benjamin Bardiaux / Ravi R Sonani / Matthijn Vos / Theis Jacobsen / Edward H Egelman / Michael Nilges / Olivera Francetic / Vladimir E Shevchik / Nadia Izadi-Pruneyre /
PubMed AbstractGram-negative bacteria employ the type II secretion system (T2SS) to transport folded protein effectors via a periplasmic helical polymer called the endopilus, composed of one major and four minor ...Gram-negative bacteria employ the type II secretion system (T2SS) to transport folded protein effectors via a periplasmic helical polymer called the endopilus, composed of one major and four minor pilin subunits. Endopili resemble type IV pili but feature a conserved calcium-binding site stabilizing their major pilins. Endopilus polymerization is coupled to substrate translocation through a dedicated outer membrane channel. We compared T2SSs from plant and human pathogens, Dickeya dadantii and Klebsiella oxytoca, respectively. Despite different ecological niches and secreted effectors, their major pilins (OutG and PulG) share >77% sequence identity. Using NMR and cryo-EM, we solved structures of calcium-bound OutG monomer, as well as OutG and PulG endopili at 3.6 Å resolution. Combining structural, mutational, and biophysical analyses with in vivo assays, we identified key determinants of secretion specificity and endopilus stability. Our findings reveal how minor sequence variations in conserved nanomachines drive functional adaptation to diverse environments.
External linksStructure / PubMed:42624105
MethodsEM (helical sym.) / NMR (solution)
Resolution3.6 Å
Structure data

75832

11me

EMDB-75832, PDB-11me:
Cryo-EM of T2SS OutG pilus
Method: EM (helical sym.) / Resolution: 3.6 Å

75833

11mf

EMDB-75833, PDB-11mf:
Cryo-EM of T2SS PulG pilus
Method: EM (helical sym.) / Resolution: 3.6 Å

PDB-29jd:
NMR Structure of the Calcium bound form of OutG, major pilin from Dickeya dadantii T2SS
Method: SOLUTION NMR

Chemicals

ChemComp-CA:
Unknown entry

Source
  • dickeya dadantii (bacteria)
  • klebsiella oxytoca (bacteria)
KeywordsPROTEIN FIBRIL / Endopilus / T2SS / PulG / PROTEIN TRANSPORT / Dickeya dadantii T2SS / major pilin / pilus / secretion

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