EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Analysis of the heterogenous structural states of the hexameric ATPase PilU of the type IV pili from Vibrio cholerae.
ジャーナル・号・ページ	Protein Sci, Vol. 35, Issue 6, Page e70609, Year 2026
掲載日	2026年5月5日
著者	Yirui Guo / Shantanu Shukla / George Minasov / Nicole L Inniss / Thomas Klose / Valerie L Tokars / Alfonso Mondragón / Zbyszek Otwinowski / Dominika Borek / Karla J F Satchell /
PubMed 要旨	Type IV pili (T4P) mediate surface motility, host interactions, and DNA uptake through cycles of extension and retraction. While the primary retraction ATPase PilT has been extensively characterized, ...Type IV pili (T4P) mediate surface motility, host interactions, and DNA uptake through cycles of extension and retraction. While the primary retraction ATPase PilT has been extensively characterized, its homolog PilU remains less well understood despite being demonstrated as a PilT-dependent retraction ATPase. Here, we determined six PilU structures by cryo-electron microscopy and x-ray crystallography. The structures reveal a homohexameric assembly stabilized by interactions between the C-terminal catalytic domain of one subunit and the N-terminal PAS-like domain of a neighboring subunit. PilU adopts multiple conformational states, exhibiting different combinations of open and closed interfaces even in the absence of nucleotide. Comparison with PilT highlights structural features that likely underlie PilU's weak ATPase activity and its dependence on PilT for function. Together, these findings provide a structural framework for understanding PilU's role within the T4P retraction machinery.
リンク	Protein Sci / PubMed:42084485 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.7 - 3.76 Å
構造データ	75296 10my EMDB-75296, PDB-10my: Single particle reconstruction of PilU from Vibrio cholerae El Tor E7946, form 1 手法: EM (単粒子) / 解像度: 3.44 Å 75297 10mz EMDB-75297, PDB-10mz: Single particle reconstruction of PilU from Vibrio cholerae El Tor E7946, form 2 手法: EM (単粒子) / 解像度: 3.68 Å 75298 10na EMDB-75298, PDB-10na: Single particle reconstruction of PilU from Vibrio cholerae El Tor E7946, form 3 手法: EM (単粒子) / 解像度: 3.49 Å 75299 10nb EMDB-75299, PDB-10nb: Single particle reconstruction of PilU from Vibrio cholerae El Tor E7946, form 4 手法: EM (単粒子) / 解像度: 3.76 Å PDB-9q6m: Crystal Structure of Vibrio cholerae PilU, a PilT-dependent Retraction ATPase - Crystal Form 1. 手法: X-RAY DIFFRACTION / 解像度: 2.95 Å PDB-9q6w: Crystal Structure of Vibrio cholerae PilU, a PilT-dependent Retraction ATPase - Crystal Form 2 手法: X-RAY DIFFRACTION / 解像度: 2.7 Å
化合物	ChemComp-HOH: WATER ChemComp-SO4: SULFATE ION
由来	vibrio cholerae (コレラ菌) vibrio cholerae o1 biovar el tor str. n16961 (コレラ菌)
キーワード	MOTOR PROTEIN / Retraction ATPase / Structural Genomics / Center for Structural Biology of Infectious Diseases / CSBID / PilU / PilT-dependent Retraction ATPase