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TitleResveratrol as a nontoxic excipient stabilizes insulin in a bioactive hexameric form.
Journal, issue, pagesJ Comput Aided Mol Des, Vol. 34, Issue 8, Page 915-927, Year 2020
Publish dateApr 9, 2020
AuthorsBani Kumar Pathak / Debajyoti Das / Sayan Bhakta / Partha Chakrabarti / Jayati Sengupta /
PubMed AbstractInsulin aggregation is the leading cause of considerable reduction in the amount of active drug molecules in liquid formulations manufactured for diabetes management. Phenolic compounds, such as ...Insulin aggregation is the leading cause of considerable reduction in the amount of active drug molecules in liquid formulations manufactured for diabetes management. Phenolic compounds, such as phenol and m-cresol, are routinely used to stabilize insulin in a hexameric form during its commercial preparation. However, long term usage of commercial insulin results in various adverse secondary responses, for which toxicity of the phenolic excipients is primarily responsible. In this study we aimed to find out a nontoxic insulin stabilizer. To that end, we have selected resveratrol, a natural polyphenol, as a prospective nontoxic insulin stabilizer because of its structural similarity with commercially used phenolic compounds. Atomic force microscopy visualization of resveratrol-treated human insulin revealed that resveratrol has a unique ability to arrest hINS in a soluble oligomeric form having discrete spherical morphology. Most importantly, resveratrol-treated insulin is nontoxic for HepG2 cells and it effectively maintains low blood glucose in a mouse model. Cryo-electron microscopy revealed 3D morphology of resveratrol-stabilized insulin that strikingly resembles crystal structures of insulin hexamer formulated with m-cresol. Significantly, we found that, in a condition inductive to amyloid fibrillation at physiological pH, resveratrol is capable of stabilizing insulin more efficiently than m-cresol. Thus, this study describes resveratrol as an effective nontoxic natural molecule that can be used for stabilizing insulin in a bioactive oligomeric form during its commercial formulation.
External linksJ Comput Aided Mol Des / PubMed:32270361
MethodsEM (single particle)
Resolution14.5 Å
Structure data

EMDB-9878: Cryo EM density map of Resveratrol-stabilized bioactive insulin oligomer
PDB-6jr3: Crystal structure of insulin hexamer fitted into cryo EM density map where each dimer was kept as rigid body
Method: EM (single particle) / Resolution: 14.5 Å

Source
  • homo sapiens (human)
KeywordsHORMONE / Insulin fibrillation / Natural polyphenols / Anti-Amyloid activity / Insulin hexamer / Bioavailability

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