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Structure paper

TitleStructural basis of nucleosome assembly by the Abo1 AAA+ ATPase histone chaperone.
Journal, issue, pagesNat Commun, Vol. 10, Issue 1, Page 5764, Year 2019
Publish dateDec 17, 2019
AuthorsCarol Cho / Juwon Jang / Yujin Kang / Hiroki Watanabe / Takayuki Uchihashi / Seung Joong Kim / Koichi Kato / Ja Yil Lee / Ji-Joon Song /
PubMed AbstractThe fundamental unit of chromatin, the nucleosome, is an intricate structure that requires histone chaperones for assembly. ATAD2 AAA+ ATPases are a family of histone chaperones that regulate ...The fundamental unit of chromatin, the nucleosome, is an intricate structure that requires histone chaperones for assembly. ATAD2 AAA+ ATPases are a family of histone chaperones that regulate nucleosome density and chromatin dynamics. Here, we demonstrate that the fission yeast ATAD2 homolog, Abo1, deposits histone H3-H4 onto DNA in an ATP-hydrolysis-dependent manner by in vitro reconstitution and single-tethered DNA curtain assays. We present cryo-EM structures of an ATAD2 family ATPase to atomic resolution in three different nucleotide states, revealing unique structural features required for histone loading on DNA, and directly visualize the transitions of Abo1 from an asymmetric spiral (ATP-state) to a symmetric ring (ADP- and apo-states) using high-speed atomic force microscopy (HS-AFM). Furthermore, we find that the acidic pore of ATP-Abo1 binds a peptide substrate which is suggestive of a histone tail. Based on these results, we propose a model whereby Abo1 facilitates H3-H4 loading by utilizing ATP.
External linksNat Commun / PubMed:31848341 / PubMed Central
MethodsEM (single particle)
Resolution3.54 - 6.9 Å
Structure data

EMDB-0800:
Abo1 structure with AAA+ and bromodomains in apo state
Method: EM (single particle) / Resolution: 6.9 Å

9870

6jpq

EMDB-9870, PDB-6jpq:
CryoEM structure of Abo1 hexamer - ADP complex
Method: EM (single particle) / Resolution: 4.44 Å

9871

6jpu

EMDB-9871, PDB-6jpu:
CryoEM structure of Abo1 hexamer - apo complex
Method: EM (single particle) / Resolution: 4.27 Å

9872

6jq0

EMDB-9872, PDB-6jq0:
CryoEM structure of Abo1 Walker B (E372Q) mutant hexamer - ATP complex
Method: EM (single particle) / Resolution: 3.54 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

Source
  • Schizosaccharomyces pombe (fission yeast)
  • Saccharomyces (fungus)
  • schizosaccharomyces pombe (strain 972 / atcc 24843) (yeast)
  • schizosaccharomyces pombe 972h- (yeast)
  • spodoptera frugiperda (fall armyworm)
KeywordsCHAPERONE / AAA+ ATPase Histone chaperone

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