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Structure paper

TitleStructures of translationally inactive mammalian ribosomes.
Journal, issue, pagesElife, Vol. 7, Year 2018
Publish dateOct 24, 2018
AuthorsAlan Brown / Matthew R Baird / Matthew Cj Yip / Jason Murray / Sichen Shao /
PubMed AbstractThe cellular levels and activities of ribosomes directly regulate gene expression during numerous physiological processes. The mechanisms that globally repress translation are incompletely understood. ...The cellular levels and activities of ribosomes directly regulate gene expression during numerous physiological processes. The mechanisms that globally repress translation are incompletely understood. Here, we use electron cryomicroscopy to analyze inactive ribosomes isolated from mammalian reticulocytes, the penultimate stage of red blood cell differentiation. We identify two types of ribosomes that are translationally repressed by protein interactions. The first comprises ribosomes sequestered with elongation factor 2 (eEF2) by SERPINE mRNA binding protein 1 (SERBP1) occupying the ribosomal mRNA entrance channel. The second type are translationally repressed by a novel ribosome-binding protein, interferon-related developmental regulator 2 (IFRD2), which spans the P and E sites and inserts a C-terminal helix into the mRNA exit channel to preclude translation. IFRD2 binds ribosomes with a tRNA occupying a noncanonical binding site, the 'Z site', on the ribosome. These structures provide functional insights into how ribosomal interactions may suppress translation to regulate gene expression.
External linksElife / PubMed:30355441 / PubMed Central
MethodsEM (single particle)
Resolution3.3 - 5.9 Å
Structure data

EMDB-9234:
Rabbit 80S ribosome with a P-site tRNA (unrotated state)
Method: EM (single particle) / Resolution: 5.9 Å

EMDB-9235:
Rabbit 80S ribosome with a P- and E-site tRNA
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-9236:
Rabbit 80S ribosome with a Z-site tRNA (unrotated state)
Method: EM (single particle) / Resolution: 4.2 Å

EMDB-9237, PDB-6mtb:
Rabbit 80S ribosome with P- and Z-site tRNAs (unrotated state)
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-9239, PDB-6mtc:
Rabbit 80S ribosome with Z-site tRNA and IFRD2 (unrotated state)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-9240, PDB-6mtd:
Rabbit 80S ribosome with eEF2 and SERBP1 (unrotated state with 40S head swivel)
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-9241:
Rabbit 80S ribosome with A/P and P/E tRNAs (rotated state)
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-9242, PDB-6mte:
Rabbit 80S ribosome with eEF2 and SERBP1 (rotated state)
Method: EM (single particle) / Resolution: 3.4 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

Source
  • oryctolagus cuniculus (rabbit)
  • unidentified (others)
  • Rabbit (rabbit)
  • Bovine (cattle)
KeywordsRIBOSOME / translation

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