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TitleStructural insights into the assembly and regulation of distinct viral capsid complexes.
Journal, issue, pagesNat Commun, Vol. 7, Page 13014, Year 2016
Publish dateOct 4, 2016
AuthorsSubir Sarker / María C Terrón / Yogesh Khandokar / David Aragão / Joshua M Hardy / Mazdak Radjainia / Manuel Jiménez-Zaragoza / Pedro J de Pablo / Fasséli Coulibaly / Daniel Luque / Shane R Raidal / Jade K Forwood /
PubMed AbstractThe assembly and regulation of viral capsid proteins into highly ordered macromolecular complexes is essential for viral replication. Here, we utilize crystal structures of the capsid protein from ...The assembly and regulation of viral capsid proteins into highly ordered macromolecular complexes is essential for viral replication. Here, we utilize crystal structures of the capsid protein from the smallest and simplest known viruses capable of autonomously replicating in animal cells, circoviruses, to establish structural and mechanistic insights into capsid morphogenesis and regulation. The beak and feather disease virus, like many circoviruses, encode only two genes: a capsid protein and a replication initiation protein. The capsid protein forms distinct macromolecular assemblies during replication and here we elucidate these structures at high resolution, showing that these complexes reverse the exposure of the N-terminal arginine rich domain responsible for DNA binding and nuclear localization. We show that assembly of these complexes is regulated by single-stranded DNA (ssDNA), and provide a structural basis of capsid assembly around single-stranded DNA, highlighting novel binding interfaces distinct from the highly positively charged N-terminal ARM domain.
External linksNat Commun / PubMed:27698405 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution2 - 4.5 Å
Structure data

EMDB-8306:
CryoEM structure of a beak and feather disease virus-like particle encapsidating ssDNA
Method: EM (single particle) / Resolution: 4.5 Å

PDB-5j09:
Crystal structure of decameric BFDV Capsid Protein
Method: X-RAY DIFFRACTION / Resolution: 2.0 Å

PDB-5j36:
Crystal structure of 60-mer BFDV Capsid Protein
Method: X-RAY DIFFRACTION / Resolution: 2.55 Å

PDB-5j37:
Crystal structure of 60-mer BFDV Capsid Protein in complex with single stranded DNA
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-PO4:
PHOSPHATE ION

Source
  • beak and feather disease virus
  • synthetic construct (others)
KeywordsVIRUS / BFDV Virus Capsid Jelly Roll / VIRUS/DNA / VIRAL PROTEIN / VIRUS-DNA complex

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